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PMID: 1064882 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mapping of the influenza virus genome: identification of the hemagglutinin and the neuraminidase genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 6 ·1976-06-00 ·Pages 2142-6

Palese P, Schulman JL

Abstract

Polyacrylamide gel electrophoresis of the RNA of influenza A/PR/8/34 (H0N1) and A/Hong Kong/8/68 (H3N2) viruses and recombinant viruses derived from them revealed that each contains eight RNA segments, the fourth of which codes for hemagglutinin. (The largest RNA of the segmented genome is counted as band 1.) The neuraminidase gene was identified as the sixth segment in the RNA pattern of influenza A/PR8 virus and as the fifth segment of A/Hong Kong virus. The molecular weights of the RNAs for the hemagglutinin and the neuraminidase genes lie in the range of 600,000-700,000.

MeSH Terms

Chromosome Mapping Electrophoresis, Polyacrylamide Gel/methods Genes Hemagglutinins, Viral Influenza A virus/analysis,enzymology Neuraminidase Orthomyxoviridae/analysis RNA, Viral/analysis Recombination, Genetic

Chemicals

Hemagglutinins, Viral RNA, Viral Neuraminidase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Palese P

Schulman J L

References (12)

12 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.73.6.2142

Published

1976-06-00

Pages

2142-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC430466

Subset

External Links

PubMed source DOI: 10.1073/pnas.73.6.2142 PMC Full Text View journal details

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Mapping of the influenza virus genome: identification of the hemagglutinin and the neuraminidase genes.

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