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PMID: 10656596 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation, partial sequencing, and expression of pathogenesis-related cDNA genes from pepper leaves infected by Xanthomonas campestris pv. vesicatoria.

Molecular plant-microbe interactions : MPMI ·Vol. 13 ·No. 1 ·2000-01-00 ·Pages 136-42

Jung HW, Hwang BK

Abstract

Specific cDNAs showing differential expression in bacteria-infected pepper leaves as opposed to healthy leaves were isolated from a pepper cDNA library from hypersensitive response (HR) lesions of leaves infected with an avirulent strain of Xanthomonas campestris pv. vesicatoria. Among a total of 282 cDNA clones tested, 36 individual cDNA genes (13%) hybridized strongly or differentially to the cDNA probes from bacteria-infected leaves. Ten Capsicum Annuum-Induced (CAI) genes encoding putative thionin, lipid transfer protein I and II, osmotin (PR-5), class I chitinase, beta-1,3-glucanase, SAR 8.2, stellacyanin, leucine-rich repeat protein, and auxin-repressed protein were identified. Two CAI genes showed little or no sequence homology to the previously sequenced plant genes. Transcripts of the CAI genes were strongly or preferentially induced in pepper tissues by infection with X. campestris pv. vesicatoria or Phytophthora capsici, and by abiotic elicitor treatment. In particular, most of the CAI genes were strongly induced in pepper tissues by ethephon and methyl jasmonate.

MeSH Terms

Acetates/pharmacology Amino Acid Sequence Blotting, Northern Capsicum/genetics,metabolism,microbiology Cyclopentanes/pharmacology Gene Library Molecular Sequence Data Organophosphorus Compounds/pharmacology Oxylipins Phytophthora/pathogenicity Plant Growth Regulators/pharmacology Plant Leaves/genetics,metabolism,microbiology Plants, Medicinal RNA, Plant/analysis Sequence Homology, Amino Acid Xanthomonas campestris/pathogenicity

Chemicals

Acetates Cyclopentanes Organophosphorus Compounds Oxylipins Plant Growth Regulators RNA, Plant methyl jasmonate ethephon

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Jung H W

Department of Agricultural Biology, Korea University, Seoul.

Hwang B K

Article Info

Journal

Molecular plant-microbe interactions : MPMI

Abbr.

Mol Plant Microbe Interact

ISSN

0894-0282

DOI

10.1094/MPMI.2000.13.1.136

Published

2000-01-00

Pages

136-42

Language

English

Region

United States

NLM ID

9107902

Subset

Databases

GENBANK

AF082705, AF082706, AF082707, AF082708, AF082709, AF082710, AF082711, AF082712, AF082713, AF082714, AF082715, AF082716, AF082717, AF082718, AF082719, AF082720, AF082721, AF082722, AF082723, AF082724, AF082725, AF082726, AF082727, AF082728, AF082729

External Links

PubMed source DOI: 10.1094/MPMI.2000.13.1.136 View journal details

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Isolation, partial sequencing, and expression of pathogenesis-related cDNA genes from pepper leaves infected by Xanthomonas campestris pv. vesicatoria.

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