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PMID: 10669741 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two novel Drosophila TAF(II)s have homology with human TAF(II)30 and are differentially regulated during development.

Molecular and cellular biology ·Vol. 20 ·No. 5 ·2000-03-00 ·Pages 1639-48

Georgieva S, Kirschner DB, Jagla T, Nabirochkina E, Hanke S, Schenkel H, de Lorenzo C, Sinha P, Jagla K, Mechler B, Tora L

Abstract

TFIID is a multiprotein complex composed of the TATA binding protein (TBP) and TBP-associated factors (TAF(II)s). The binding of TFIID to the promoter is the first step of RNA polymerase II preinitiation complex assembly on protein-coding genes. Yeast (y) and human (h) TFIID complexes contain 10 to 13 TAF(II)s. Biochemical studies suggested that the Drosophila (d) TFIID complexes contain only eight TAF(II)s, leaving a number of yeast and human TAF(II)s (e.g., hTAF(II)55, hTAF(II)30, and hTAF(II)18) without known Drosophila homologues. We demonstrate that Drosophila has not one but two hTAF(II)30 homologues, dTAF(II)16 and dTAF(II)24, which are encoded by two adjacent genes. These two genes are localized in a head-to-head orientation, and their 5' extremities overlap. We show that these novel dTAF(II)s are expressed and that they are both associated with TBP and other bona fide dTAF(II)s in dTFIID complexes. dTAF(II)24, but not dTAF(II)16, was also found to be associated with the histone acetyltransferase (HAT) dGCN5. Thus, dTAF(II)16 and dTAF(II)24 are functional homologues of hTAF(II)30, and this is the first demonstration that a TAF(II)-GCN5-HAT complex exists in Drosophila. The two dTAF(II)s are differentially expressed during embryogenesis and can be detected in both nuclei and cytoplasm of the cells. These results together indicate that dTAF(II)16 and dTAF(II)24 may have similar but not identical functions.

MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA-Binding Proteins/genetics Drosophila/embryology,genetics Drosophila Proteins Gene Expression Regulation, Developmental Genes, Insect Humans Insect Proteins/genetics Molecular Sequence Data Sequence Analysis Sequence Homology, Amino Acid TATA-Binding Protein Associated Factors Transcription Factor TFIID Transcription Factors/genetics
Chemicals
DNA-Binding Proteins Drosophila Proteins Insect Proteins TAF10 protein, human TATA-Binding Protein Associated Factors Taf10 protein, Drosophila Taf10b protein, Drosophila Transcription Factor TFIID Transcription Factors
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Georgieva S
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, F-67404 Illkirch Cedex, CU de Strasbourg, France.
Kirschner D B
Jagla T
Nabirochkina E
Hanke S
Schenkel H
de Lorenzo C
Sinha P
Jagla K
Mechler B
Tora L
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.20.5.1639-1648.2000
Published
2000-03-00
Pages
1639-48
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC85347
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.20.5.1639-1648.2000 PMC Full Text View journal details
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