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PMID: 10675337 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The novel coactivator C1 (HCF) coordinates multiprotein enhancer formation and mediates transcription activation by GABP.

The EMBO journal ·Vol. 19 ·No. 4 ·2000-02-15 ·Pages 683-90

Vogel JL, Kristie TM

Abstract

Transcription of the herpes simplex virus 1 (HSV-1) immediate early (IE) genes is determined by multiprotein enhancer complexes. The core enhancer assembly requires the interactions of the POU-homeodomain protein Oct-1, the viral transactivator alphaTIF and the cellular factor C1 (HCF). In this context, the C1 factor interacts with each protein to assemble the stable enhancer complex. In addition, the IE enhancer cores contain adjacent binding sites for other cellular transcription factors such as Sp1 and GA-binding protein (GABP). In this study, a direct interaction of the C1 factor with GABP is demonstrated, defining the C1 factor as the critical coordinator of the enhancer complex assembly. In addition, mutations that reduce the GABP transactivation potential also impair the C1-GABP interaction, indicating that the C1 factor functions as a novel coactivator of GABP-mediated transcription. The interaction and coordinated assembly of the enhancer proteins by the C1 factor may be critical for the regulation of the HSV lytic-latent cycle.

MeSH Terms
Amino Acid Sequence Base Sequence Binding Sites/genetics Cell Line DNA Primers/genetics DNA-Binding Proteins/genetics,metabolism Enhancer Elements, Genetic GA-Binding Protein Transcription Factor Genes, Immediate-Early HeLa Cells Herpesvirus 1, Human/genetics,physiology Host Cell Factor C1 Humans Molecular Sequence Data Protein Structure, Tertiary Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Transcription Factors/genetics,metabolism Transcriptional Activation Two-Hybrid System Techniques
Chemicals
DNA Primers DNA-Binding Proteins GA-Binding Protein Transcription Factor HCFC1 protein, human Host Cell Factor C1 Proteins Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Vogel J L
Laboratory of Viral Diseases, National Institutes of Health, Building 4, Room 133, 4 Center Drive, Bethesda, MD 20892, USA.
Kristie T M
References (53)
53 references, click to expand
  1. Convergence of Ets- and notch-related structural motifs in a heteromeric DNA binding complex.
    Science. 1991 Aug 16;253(5021):762-8 PMID: 1876833
  2. A cellular factor binds to the herpes simplex virus type 1 transactivator Vmw65 and is required for Vmw65-dependent protein-DNA complex assembly with Oct-1.
    Mol Cell Biol. 1990 Sep;10(9):4974-7 PMID: 2167442
  3. Recognition of the surface of a homeo domain protein.
    Genes Dev. 1992 Nov;6(11):2047-57 PMID: 1358755
  4. Mapping of a major surface-exposed site in herpes simplex virus protein Vmw65 to a region of direct interaction in a transcription complex assembly.
    J Virol. 1993 Feb;67(2):852-62 PMID: 8380468
  5. cDNA cloning of transcription factor E4TF1 subunits with Ets and notch motifs.
    Mol Cell Biol. 1993 Mar;13(3):1385-91 PMID: 8441384
  6. A sequence-specific DNA-binding protein recognising a GA-rich element cooperates with Oct-1 at the herpes simplex virus type 1 IE3 promoter.
    Intervirology. 1992;34(2):74-85 PMID: 1338062
  7. Identity of GABP with NRF-2, a multisubunit activator of cytochrome oxidase expression, reveals a cellular role for an ETS domain activator of viral promoters.
    Genes Dev. 1993 Mar;7(3):380-92 PMID: 8383622
  8. Purification of the cellular C1 factor required for the stable recognition of the Oct-1 homeodomain by the herpes simplex virus alpha-trans-induction factor (VP16).
    J Biol Chem. 1993 Mar 25;268(9):6525-34 PMID: 8454622
  9. The VP16 accessory protein HCF is a family of polypeptides processed from a large precursor protein.
    Cell. 1993 Jul 16;74(1):115-25 PMID: 8392914
  10. Crystal structure of the Oct-1 POU domain bound to an octamer site: DNA recognition with tethered DNA-binding modules.
    Cell. 1994 Apr 8;77(1):21-32 PMID: 8156594
  11. Transcriptional activation by herpes simplex virus type 1 VP16 in vitro and its inhibition by oligopeptides.
    Mol Cell Biol. 1994 May;14(5):3484-93 PMID: 8164693
  12. Combinatorial control of transcription: the herpes simplex virus VP16-induced complex.
    Cold Spring Harb Symp Quant Biol. 1993;58:167-78 PMID: 7956027
  13. Molecular and genetic characterization of GABP beta.
    Genes Dev. 1994 Aug 1;8(15):1853-65 PMID: 7958862
  14. Four structurally distinct, non-DNA-binding subunits of human nuclear respiratory factor 2 share a conserved transcriptional activation domain.
    Mol Cell Biol. 1995 Jan;15(1):102-11 PMID: 7799916
  15. Positive and negative regulation at the herpes simplex virus ICP4 and ICP0 TAATGARAT motifs.
    Virology. 1995 Feb 20;207(1):107-16 PMID: 7871718
  16. The cellular C1 factor of the herpes simplex virus enhancer complex is a family of polypeptides.
    J Biol Chem. 1995 Mar 3;270(9):4387-94 PMID: 7876203
  17. GABP and PU.1 compete for binding, yet cooperate to increase CD18 (beta 2 leukocyte integrin) transcription.
    J Biol Chem. 1995 Oct 6;270(40):23627-33 PMID: 7559529
  18. The HCF repeat is an unusual proteolytic cleavage signal.
    Genes Dev. 1995 Oct 15;9(20):2445-58 PMID: 7590226
  19. Raf-1 kinase targets GA-binding protein in transcriptional regulation of the human immunodeficiency virus type 1 promoter.
    J Virol. 1996 Apr;70(4):2260-8 PMID: 8642652
  20. Characterization of a cellular factor which interacts functionally with Oct-1 in the assembly of a multicomponent transcription complex.
    Nucleic Acids Res. 1990 Dec 11;18(23):6871-80 PMID: 2175881
  21. The oct-1 homeo domain contacts only part of the octamer sequence and full oct-1 DNA-binding activity requires the POU-specific domain.
    Genes Dev. 1990 Nov;4(11):1964-74 PMID: 1980478
  22. Interactions of the Oct-1 POU subdomains with specific DNA sequences and with the HSV alpha-trans-activator protein.
    Genes Dev. 1990 Dec;4(12B):2383-96 PMID: 1980658
  23. Binding of the Ets factor GA-binding protein to an upstream site in the factor IX promoter is a critical event in transactivation.
    Mol Cell Biol. 1996 May;16(5):1929-35 PMID: 8628259
  24. GABP mediates insulin-increased prolactin gene transcription.
    J Biol Chem. 1996 May 3;271(18):10425-8 PMID: 8631833
  25. Nuclear respiratory factors 1 and 2 utilize similar glutamine-containing clusters of hydrophobic residues to activate transcription.
    Mol Cell Biol. 1996 Oct;16(10):5708-16 PMID: 8816484
  26. A single-point mutation in HCF causes temperature-sensitive cell-cycle arrest and disrupts VP16 function.
    Genes Dev. 1997 Mar 15;11(6):726-37 PMID: 9087427
  27. Interdigitated residues within a small region of VP16 interact with Oct-1, HCF, and DNA.
    Mol Cell Biol. 1997 Jul;17(7):3937-46 PMID: 9199328
  28. Luman, a new member of the CREB/ATF family, binds to herpes simplex virus VP16-associated host cellular factor.
    Mol Cell Biol. 1997 Sep;17(9):5117-26 PMID: 9271389
  29. VP16 targets an amino-terminal domain of HCF involved in cell cycle progression.
    Mol Cell Biol. 1997 Oct;17(10):6139-46 PMID: 9315674
  30. Concerted activity of host cell factor subregions in promoting stable VP16 complex assembly and preventing interference by the acidic activation domain.
    Mol Cell Biol. 1997 Dec;17(12):7108-18 PMID: 9372942
  31. Viral mimicry: common mode of association with HCF by VP16 and the cellular protein LZIP.
    Genes Dev. 1997 Dec 1;11(23):3122-7 PMID: 9389645
  32. The structure of GABPalpha/beta: an ETS domain- ankyrin repeat heterodimer bound to DNA.
    Science. 1998 Feb 13;279(5353):1037-41 PMID: 9461436
  33. Regulation of human B19 parvovirus promoter expression by hGABP (E4TF1) transcription factor.
    J Biol Chem. 1998 Apr 3;273(14):8287-93 PMID: 9525935
  34. The GABP-responsive element of the interleukin-2 enhancer is regulated by JNK/SAPK-activating pathways in T lymphocytes.
    J Biol Chem. 1998 Apr 24;273(17):10112-9 PMID: 9553058
  35. Sp1 cooperates with the ets transcription factor, GABP, to activate the CD18 (beta2 leukocyte integrin) promoter.
    J Biol Chem. 1998 May 22;273(21):13097-103 PMID: 9582348
  36. The herpesvirus transactivator VP16 mimics a human basic domain leucine zipper protein, luman, in its interaction with HCF.
    J Virol. 1998 Aug;72(8):6291-7 PMID: 9658067
  37. An enhancer located between the neutrophil elastase and proteinase 3 promoters is activated by Sp1 and an Ets factor.
    J Biol Chem. 1999 Jan 8;274(2):1085-91 PMID: 9873055
  38. HCF-dependent nuclear import of VP16.
    EMBO J. 1999 Jan 15;18(2):480-9 PMID: 9889203
  39. Nuclear localization of the C1 factor (host cell factor) in sensory neurons correlates with reactivation of herpes simplex virus from latency.
    Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1229-33 PMID: 9990006
  40. Analysis of functional domains of the host cell factor involved in VP16 complex formation.
    J Biol Chem. 1999 Jun 4;274(23):16437-43 PMID: 10347205
  41. Structural features of the herpes simplex virus alpha gene 4, 0, and 27 promoter-regulatory sequences which confer alpha regulation on chimeric thymidine kinase genes.
    J Virol. 1982 Dec;44(3):939-49 PMID: 6294341
  42. Separation of sequences defining basal expression from those conferring alpha gene recognition within the regulatory domains of herpes simplex virus 1 alpha genes.
    Proc Natl Acad Sci U S A. 1984 Jul;81(13):4065-9 PMID: 6330737
  43. Sp1 binds to promoter sequences and activates herpes simplex virus 'immediate-early' gene transcription in vitro.
    Nature. 1985 Sep 12-18;317(6033):179-82 PMID: 2993923
  44. Binding of the virion protein mediating alpha gene induction in herpes simplex virus 1-infected cells to its cis site requires cellular proteins.
    Proc Natl Acad Sci U S A. 1987 Oct;84(20):7061-5 PMID: 2823252
  45. A complex formed between cell components and an HSV structural polypeptide binds to a viral immediate early gene regulatory DNA sequence.
    Cell. 1988 Feb 12;52(3):425-34 PMID: 2830986
  46. Herpes simplex virus regulatory elements and the immunoglobulin octamer domain bind a common factor and are both targets for virion transactivation.
    Cell. 1988 Feb 12;52(3):435-45 PMID: 2830987
  47. A herpesvirus trans-activating protein interacts with transcription factor OTF-1 and other cellular proteins.
    Proc Natl Acad Sci U S A. 1988 Sep;85(17):6347-51 PMID: 2842768
  48. Evidence of DNA: protein interactions that mediate HSV-1 immediate early gene activation by VP16.
    Genes Dev. 1988 Jun;2(6):730-42 PMID: 2843426
  49. The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain.
    Genes Dev. 1988 Dec;2(12A):1582-99 PMID: 2905684
  50. The Oct-1 homoeodomain directs formation of a multiprotein-DNA complex with the HSV transactivator VP16.
    Nature. 1989 Oct 19;341(6243):624-30 PMID: 2571937
  51. The octamer-binding proteins form multi-protein--DNA complexes with the HSV alpha TIF regulatory protein.
    EMBO J. 1989 Dec 20;8(13):4229-38 PMID: 2556266
  52. Purification of a set of cellular polypeptides that bind to the purine-rich cis-regulatory element of herpes simplex virus immediate early genes.
    Genes Dev. 1989 Sep;3(9):1372-83 PMID: 2558055
  53. The herpes simplex virus trans-activator VP16 recognizes the Oct-1 homeo domain: evidence for a homeo domain recognition subdomain.
    Genes Dev. 1991 Dec;5(12B):2555-66 PMID: 1684335
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.4.683
Published
2000-02-15
Pages
683-90
Language
English
Region
England
NLM ID
8208664
PMCID
PMC305606
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/19.4.683 PMC Full Text View journal details
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