Home LiteratureArticle Details
PMID: 1067606 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Interferon, double-stranded RNA, and protein phosphorylation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 9 ·1976-09-00 ·Pages 3107-11

Lebleu B, Sen GC, Shaila S, Cabrer B, Lengyel P

Abstract

We reported earlier that the addition of double-stranded RNA and ATP increases the endonuclease activity more in an extract of Ehrlich ascites tumor cells which have been treated with an interferon preparation than in a comparable extract from control cells. We report here that the addition of double-stranded RNA to an extract from Ehrlich ascites tumor cells which have been treated with an interferon preparation [or with the interferon inducer poly(I)-poly(C)] promotes the phosphorylation by [gamma-32P]ATP of at least two proteins: P1 (molecular weight of 64,000) and P2 (molecular weight of 37,000). Double-stranded RNA also promotes the phosphorylation of at least one (i.e., P1) of these two proteins in an extract from cells which have not been treated with interferon, but the extent of phosphorylation is much smaller. Double-stranded RNA which has been degraded by RNase III, or DNA, does not promote the phosphorylation.

MeSH Terms
Adenosine Triphosphate/metabolism Animals Carcinoma, Ehrlich Tumor DNA, Viral/pharmacology Dose-Response Relationship, Drug Humans Interferons/pharmacology Mice Molecular Weight Neoplasm Proteins/metabolism Phosphoproteins/biosynthesis Poly I-C/pharmacology RNA, Viral/pharmacology Reoviridae Species Specificity
Chemicals
DNA, Viral Neoplasm Proteins Phosphoproteins RNA, Viral Adenosine Triphosphate Interferons Poly I-C
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lebleu B
Sen G C
Shaila S
Cabrer B
Lengyel P
References (24)
24 references, click to expand
  1. Increased sensitivity of cell-free protein synthesis to double-stranded RNA after interferon treatment.
    Nature. 1974 Jul 5;250(461):57-9 PMID: 4366491
  2. Inhibitors of protein synthesis.
    FEBS Lett. 1974 Mar 23;40(0):suppl:S63-84 PMID: 4604618
  3. Selective inhibition of viral protein accumulation in interferon-treated cells; nondiscriminate inhibition of the translation of added viral and cellular messenger RNAs in their extracts.
    Virology. 1974 Jan;57(1):49-63 PMID: 4206536
  4. Ribonuclease 3 does not degrade deoxyribonucleic acid-ribonucleic acid hybrids.
    J Biol Chem. 1974 Feb 25;249(4):1314-6 PMID: 4592261
  5. Interferon assay based on the inhibition of double-stranded reovirus RNA accumulation in mouse L cells.
    J Gen Virol. 1973 Apr;19(1):81-7 PMID: 4360633
  6. A protein synthesizing system from interferon-treated cells that discriminates between cellular and viral messenger RNAs.
    Virology. 1974 Apr;58(2):476-91 PMID: 4820887
  7. Translation of in vitro synthesized reovirus messenger RNAs into proteins of the size of reovirus capsid proteins in a mouse L cell extract.
    Biochem Biophys Res Commun. 1972 Mar 10;46(5):1816-23 PMID: 5062717
  8. Quantitative aspects of interferon-induced plaque reduction: kinetics of interferon action.
    Virology. 1972 May;48(2):425-32 PMID: 4337029
  9. Mechanism of interferon action: inhibition of viral messenger ribonucleic acid translation in L-cell extracts.
    J Virol. 1972 Dec;10(6):1184-98 PMID: 4345494
  10. Correlation between the antiviral effect of interferon treatment and the inhibition of in vitro mRNA translation in noninfected L cells.
    J Virol. 1973 Sep;12(3):421-30 PMID: 4355848
  11. Interferon treatment of Ehrlich ascites tumor cells: effects on exogenous mRNA translation and tRNA inactivation in the cell extract.
    J Virol. 1975 Jan;17(1):191-203 PMID: 173882
  12. Release of the inhibition of messenger RNA translation in extracts of interferon-treated Ehrlich ascites tumor cells by added transfer RNA.
    Biochem Biophys Res Commun. 1974 Apr 8;57(3):763-70 PMID: 4363942
  13. Formation and characteristics of reovirus subviral particles in interferon-treated mouse L cells.
    Nucleic Acids Res. 1976 Mar;3(3):581-98 PMID: 58408
  14. Protein phosphorylation.
    Annu Rev Biochem. 1975;44:831-87 PMID: 166607
  15. Control of protein synthesis in reticulocyte lysates: effects of 3':5'-cyclic AMP, ATP, and GTP on inhibitions induced by hemedeficiency, double-stranded RNA, and a reticulocyte translationa inhibitor.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1112-6 PMID: 177976
  16. Possible role for cyclic nucleotides and phosphorylated membrane proteins in postsynaptic actions of neurotransmitters.
    Nature. 1976 Mar 11;260(5547):101-8 PMID: 176592
  17. Association of a cyclic AMP-dependent protein kinase with a purified translational inhibitor isolated from hemin-deficient rabbit reticulocyte lysates.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):4849-53 PMID: 174078
  18. Purification and properties of ribonuclease III from Escherichia coli.
    J Biol Chem. 1968 Jan 10;243(1):82-91 PMID: 4865702
  19. Increased endonuclease activity in an extract from mouse Ehrlich ascites tumor cells which had been treated with a partially purified interferon preparation: dependence of double-stranded RNA;.
    Biochem Biophys Res Commun. 1976 Mar 8;69(1):114-22 PMID: 1259747
  20. A cell free system from HeLa cells active in initiation of protein synthesis.
    Biochemistry. 1975 Dec 2;14(24):5315-21 PMID: 1191638
  21. Control of protein synthesis in reticulocyte lysates: the effect of nucleotide triphosphates on formation of the translational repressor.
    Biochem Biophys Res Commun. 1975 Nov 3;67(1):366-75 PMID: 1201028
  22. Inhibition of reovirus messenger RNA methylation in extracts of interferon-treated Ehrlich ascites tumor cells.
    Biochem Biophys Res Commun. 1975 Jul 8;65(1):427-34 PMID: 1147999
  23. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  24. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.9.3107
Published
1976-09-00
Pages
3107-11
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430945
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.9.3107 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2