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PMID: 10681364 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A convenient assay for estimating the possible involvement of efflux of fluoroquinolones by Streptococcus pneumoniae and Staphylococcus aureus: evidence for diminished moxifloxacin, sparfloxacin, and trovafloxacin efflux.

Antimicrobial agents and chemotherapy ·Vol. 44 ·No. 3 ·2000-03-00 ·Pages 798-801

Beyer R, Pestova E, Millichap JJ, Stosor V, Noskin GA, Peterson LR

Abstract

We developed a simplified assay for estimating efflux by measuring the effect of reserpine on the growth of Streptococcus pneumoniae and Staphylococcus aureus over 7 h. Reserpine enhanced ciprofloxacin and levofloxacin 17 to 68%. The hydrophobic drug trovafloxacin and the drug moxifloxacin, with a bulky C-7 substituent but hydrophilicity similar to that of levofloxacin, showed little (0 to 11%) reserpine-enhancing effect. The ease of resistant mutant strain selection correlated with efflux susceptibility.

MeSH Terms
Anti-Infective Agents/metabolism,pharmacology Aza Compounds Drug Resistance, Microbial Fluoroquinolones Microbial Sensitivity Tests Moxifloxacin Naphthyridines/metabolism,pharmacology Quinolines Reserpine/pharmacology Staphylococcus aureus/drug effects,genetics,metabolism Streptococcus pneumoniae/drug effects,genetics,metabolism
Chemicals
Anti-Infective Agents Aza Compounds Fluoroquinolones Naphthyridines Quinolines Reserpine trovafloxacin sparfloxacin Moxifloxacin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Beyer R
Department of Pathology, Division of Microbiology, Northwestern University Medical School, Chicago, Illinois 60611, USA.
Pestova E
Millichap J J
Stosor V
Noskin G A
Peterson L R
References (24)
24 references, click to expand
  1. Destruction of low efficiency markers is a slow process occurring at a heteroduplex stage of transformation.
    Mol Gen Genet. 1974;128(4):283-90 PMID: 4150368
  2. Contribution of topoisomerase IV and DNA gyrase mutations in Streptococcus pneumoniae to resistance to novel fluoroquinolones.
    Antimicrob Agents Chemother. 1999 Aug;43(8):2000-4 PMID: 10428926
  3. Mechanisms of fluoroquinolone resistance in Staphylococcus aureus.
    J Infect Dis. 1991 May;163(5):1080-6 PMID: 1850442
  4. A comparison of methods used for measuring the accumulation of quinolones by Enterobacteriaceae, Pseudomonas aeruginosa and Staphylococcus aureus.
    J Antimicrob Chemother. 1991 Nov;28(5):639-53 PMID: 1663928
  5. Mechanism of action of sparfloxacin against and mechanism of resistance in gram-negative and gram-positive bacteria.
    Antimicrob Agents Chemother. 1991 Nov;35(11):2423-7 PMID: 1666499
  6. Fluoroquinolone resistance protein NorA of Staphylococcus aureus is a multidrug efflux transporter.
    Antimicrob Agents Chemother. 1993 Jan;37(1):128-9 PMID: 8431010
  7. Mutants of the Bacillus subtilis multidrug transporter Bmr with altered sensitivity to the antihypertensive alkaloid reserpine.
    J Biol Chem. 1993 May 25;268(15):11086-9 PMID: 8098708
  8. Efflux-mediated fluoroquinolone resistance in Staphylococcus aureus.
    Antimicrob Agents Chemother. 1993 May;37(5):1086-94 PMID: 8517696
  9. Quinolone resistance mediated by norA: physiologic characterization and relationship to flqB, a quinolone resistance locus on the Staphylococcus aureus chromosome.
    Antimicrob Agents Chemother. 1994 Jun;38(6):1345-55 PMID: 8092836
  10. Inducible NorA-mediated multidrug resistance in Staphylococcus aureus.
    Antimicrob Agents Chemother. 1995 Dec;39(12):2650-5 PMID: 8592996
  11. Hydrophilicity of quinolones is not an exclusive factor for decreased activity in efflux-mediated resistant mutants of Staphylococcus aureus.
    Antimicrob Agents Chemother. 1996 Aug;40(8):1835-42 PMID: 8843290
  12. Inhibition of the multidrug transporter NorA prevents emergence of norfloxacin resistance in Staphylococcus aureus.
    Antimicrob Agents Chemother. 1996 Nov;40(11):2673-4 PMID: 8913490
  13. Proton-dependent multidrug efflux systems.
    Microbiol Rev. 1996 Dec;60(4):575-608 PMID: 8987357
  14. Apparent involvement of a multidrug transporter in the fluoroquinolone resistance of Streptococcus pneumoniae.
    Antimicrob Agents Chemother. 1997 Jun;41(6):1396-8 PMID: 9174208
  15. Natural functions of bacterial multidrug transporters.
    Trends Microbiol. 1997 Aug;5(8):309-13 PMID: 9263408
  16. Active efflux as a mechanism of resistance to ciprofloxacin in Streptococcus pneumoniae.
    Antimicrob Agents Chemother. 1997 Sep;41(9):1973-8 PMID: 9303396
  17. The effect of reserpine, an inhibitor of multi-drug efflux pumps, on the in-vitro susceptibilities of fluoroquinolone-resistant strains of Streptococcus pneumoniae to norfloxacin.
    J Antimicrob Chemother. 1997 Sep;40(3):458-60 PMID: 9338508
  18. Prevalence of a putative efflux mechanism among fluoroquinolone-resistant clinical isolates of Streptococcus pneumoniae.
    Antimicrob Agents Chemother. 1998 Aug;42(8):2032-5 PMID: 9687402
  19. Bacterial topoisomerases, anti-topoisomerases, and anti-topoisomerase resistance.
    Clin Infect Dis. 1998 Aug;27 Suppl 1:S54-63 PMID: 9710672
  20. Identification of an efflux pump gene, pmrA, associated with fluoroquinolone resistance in Streptococcus pneumoniae.
    Antimicrob Agents Chemother. 1999 Jan;43(1):187-9 PMID: 9869592
  21. Quinolone resistance by mutations in chromosomal gyrase genes. Just the tip of the iceberg?
    J Antimicrob Chemother. 1998 Dec;42(6):683-8 PMID: 10052889
  22. Inhibition of the emergence of ciprofloxacin resistance in Streptococcus pneumoniae by the multidrug efflux inhibitor reserpine.
    Antimicrob Agents Chemother. 1999 Apr;43(4):988-9 PMID: 10103220
  23. Decreased susceptibility of Streptococcus pneumoniae to fluoroquinolones in Canada. Canadian Bacterial Surveillance Network.
    N Engl J Med. 1999 Jul 22;341(4):233-9 PMID: 10413735
  24. Nucleotide sequence and characterization of the Staphylococcus aureus norA gene, which confers resistance to quinolones.
    J Bacteriol. 1990 Dec;172(12):6942-9 PMID: 2174864
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.44.3.798-801.2000
Published
2000-03-00
Pages
798-801
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC89772
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.44.3.798-801.2000 PMC Full Text View journal details
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