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PMID: 10691129 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A large scale structural analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags.

DNA research : an international journal for rapid publication of reports on genes and genomes ·Vol. 6 ·No. 6 ·1999-12-31 ·Pages 369-73

Asamizu E, Nakamura Y, Sato S, Fukuzawa H, Tabata S

Abstract

To understand genetic information carried in a unicellular green alga, Chlamydomonas reinhardtii, normalized and size-selected cDNA libraries were constructed from cells at photoautotrophic growth, and a total of 11,571 5'-end sequence tags were established. These sequences were grouped into 3433 independent EST species. Similarity search against the public non-redundant protein database indicated that 817 groups showed significant similarity to registered sequences, of which 140 were of previously identified C. reinhardtii genes, but the remaining 2616 species were novel sequences. The coverage of full-length protein coding regions was estimated to be over 60%. These cDNA clones and EST sequence information will provide a powerful source for the future genome-wide functional analysis of uncharacterized genes.

MeSH Terms

Animals Base Sequence Chlamydomonas reinhardtii/genetics DNA, Complementary Databases, Factual Expressed Sequence Tags Gene Library Molecular Sequence Data

Chemicals

DNA, Complementary

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Asamizu E

Kazusa DNA Research Institute, Kisarazu, Chiba, Japan.

Nakamura Y

Sato S

Fukuzawa H

Tabata S

Article Info

Journal

DNA research : an international journal for rapid publication of reports on genes and genomes

Abbr.

DNA Res

ISSN

1340-2838

DOI

10.1093/dnares/6.6.369

Published

1999-12-31

Pages

369-73

Language

English

Region

England

NLM ID

9423827

Subset

Databases

GENBANK

AV386458, AV386459, AV386460, AV386461, AV386462, AV386463, AV386464, AV386465, AV386466, AV386467, AV386468, AV386469, AV386470, AV386471, AV386472, AV386473, AV386474, AV386475, AV386476, AV386477, AV386478, AV386479, AV386480, AV386481, AV386482, AV386483, AV386484, AV386485, AV386486, AV386487

External Links

PubMed source DOI: 10.1093/dnares/6.6.369 View journal details

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A large scale structural analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags.

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