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PMID: 1069297 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Differential labeling of the cell surface of single ciliary ganglion neurons in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 11 ·1976-11-00 ·Pages 4100-4

Wessells NK, Nuttall RP, Wrenn JT, Johnson S

Abstract

Cationic ferritin binds in a time and concentration dependent manner to all surfaces of ciliary ganglion neurons in culture except "mounds" and "veils". In chase experiments, bound ferritin clears from the cells surfaces and forms larger and larger patches, even at low temperatures. Binding of cationic ferritin is inhibited by poly-L-lysine, potentiated by poly-L-glutamate, and not affected by neruaminidase (acylneuraminyl hydrolase, EC 3.2.1.18), hyaluronidase (hyaluronoglucosidase, hyaluronate 4-glycanhydrolase, EC 3.2.1.35), or chondroitin ABC lyase (EC 4.2.2.4).

MeSH Terms

Ammonium Chloride/pharmacology Animals Axons/physiology,ultrastructure Binding Sites Chick Embryo Ferritins/metabolism Ganglia/cytology Kinetics Lysine/pharmacology Microscopy, Electron Motor Neurons/metabolism,physiology,ultrastructure Neuraminidase

Chemicals

Ammonium Chloride Ferritins Neuraminidase Lysine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Wessells N K

Nuttall R P

Wrenn J T

Johnson S

References (11)

11 references, click to expand

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Fine structure of nerve fibers and growth cones of isolated sympathetic neurons in culture.
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Notochordal stimulation of in vitro somite chondrogenesis before and after enzymatic removal of perinotochordal materials.
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The interactions of lectins with animal cell surfaces.
Int Rev Cytol. 1974;39:89-190 PMID: 4611947
Freeze-fracturing of nerve growth cones and young fibers. A study of developing plasma membrane.
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Thorotrast uptake and transit in embryonic glia, heart fibroblasts and neurons in vitro.
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Glutaraldehyde induced alterations of membrane anionic sites.
Biochim Biophys Acta. 1976 Apr 5;426(4):772-5 PMID: 816378
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.73.11.4100

Published

1976-11-00

Pages

4100-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC431342

Subset

External Links

PubMed source DOI: 10.1073/pnas.73.11.4100 PMC Full Text View journal details

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Differential labeling of the cell surface of single ciliary ganglion neurons in vitro.

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