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PMID: 10698801 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transformation of Acinetobacter sp. strain BD413(pFG4DeltanptII) with transgenic plant DNA in soil microcosms and effects of kanamycin on selection of transformants.

Applied and environmental microbiology ·Vol. 66 ·No. 3 ·2000-03-00 ·Pages 1237-42

Nielsen KM, van Elsas JD, Smalla K

Abstract

Here we show that horizontal transfer of DNA, extracted from transgenic sugar beets, to bacteria, based on homologous recombination, can occur in soil. Restoration of a 317-bp-deleted nptII gene in Acinetobacter sp. strain BD413(pFG4) cells incubated in sterile soil microcosms was detected after addition of nutrients and transgenic plant DNA encoding a functional nptII gene conferring bacterial kanamycin resistance. Selective effects of the addition of kanamycin on the population dynamics of Acinetobacter sp. cells in soil were found, and high concentrations of kanamycin reduced the CFU of Acinetobacter sp. cells from 10(9) CFU/g of soil to below detection. In contrast to a chromosomal nptII-encoded kanamycin resistance, the pFG4-generated resistance was found to be unstable over a 31-day incubation period in vitro.

MeSH Terms
Acinetobacter/genetics Chenopodiaceae DNA, Plant/genetics Kanamycin/pharmacology Kanamycin Resistance/genetics Phosphotransferases (Alcohol Group Acceptor)/genetics Plants, Genetically Modified/genetics Selection, Genetic Soil Microbiology Transformation, Bacterial
Chemicals
DNA, Plant Kanamycin Phosphotransferases (Alcohol Group Acceptor)
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nielsen K M
Unigen and Department of Botany, Norwegian University of Science and Technology, 7491 Trondheim, Norway. [email protected]
van Elsas J D
Smalla K
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.66.3.1237-1242.2000
Email
[email protected]
Published
2000-03-00
Pages
1237-42
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC91972
Subset
IM
External Links
PubMed source DOI: 10.1128/AEM.66.3.1237-1242.2000 PMC Full Text View journal details
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