Home LiteratureArticle Details
PMID: 10698922 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Review

The importance of aquaporin water channel protein structures.

The EMBO journal ·Vol. 19 ·No. 5 ·2000-03-01 ·Pages 800-6

Engel A, Fujiyoshi Y, Agre P

Abstract

The history of the water channel and recent structural and functional analyses of aquaporins are reviewed. These ubiquitous channels are important for bacteria, plants and animals, exhibit a pronounced sequence homology and share functional as well as structural similarities. Aquaporins allow water or small specific solutes to pass unhindered, but block the passage of ions to prevent dissipation of the transmembrane potential. Besides advances in structure determination, recent experiments suggest that many of these channels are regulated by pH variations, phosphorylation and binding of auxiliary proteins.

MeSH Terms
Animals Aquaporins/chemistry,metabolism Humans Protein Conformation Structure-Activity Relationship
Chemicals
Aquaporins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Engel A
M.E.Müller-Institute for Microscopy at the Biozentrum, University of Basel, CH-4056, Switzerland. [email protected]
Fujiyoshi Y
Agre P
References (52)
52 references, click to expand
  1. Purified lens major intrinsic protein (MIP) forms highly ordered tetragonal two-dimensional arrays by reconstitution.
    J Mol Biol. 1998 Jun 19;279(4):855-64 PMID: 9642066
  2. The three-dimensional structure of aquaporin-1.
    Nature. 1997 Jun 5;387(6633):624-7 PMID: 9177353
  3. Voltage and pH-induced channel closure of porin OmpF visualized by atomic force microscopy.
    J Mol Biol. 1999 Jan 29;285(4):1347-51 PMID: 9917378
  4. The structure of aquaporin-1 at 4.5-A resolution reveals short alpha-helices in the center of the monomer.
    J Struct Biol. 1999 Dec 1;128(1):34-43 PMID: 10600556
  5. Kinetics of DNA hydration.
    J Mol Biol. 1997 Apr 25;268(1):118-36 PMID: 9149146
  6. Switch from an aquaporin to a glycerol channel by two amino acids substitution.
    J Biol Chem. 1999 Mar 12;274(11):6817-9 PMID: 10066730
  7. Molecular structure of the water channel through aquaporin CHIP. The hourglass model.
    J Biol Chem. 1994 May 20;269(20):14648-54 PMID: 7514176
  8. Thermal denaturation of ribonuclease A characterized by water 17O and 2H magnetic relaxation dispersion.
    Biochemistry. 1998 Jun 30;37(26):9595-604 PMID: 9649343
  9. The three-dimensional structure of human erythrocyte aquaporin CHIP.
    EMBO J. 1994 Jul 1;13(13):2985-93 PMID: 7518771
  10. Structure of the water channel AqpZ from Escherichia coli revealed by electron crystallography.
    J Mol Biol. 1999 Sep 3;291(5):1181-90 PMID: 10518953
  11. Molecular design of aquaporin-1 water channel as revealed by electron crystallography.
    Nat Struct Biol. 1997 Apr;4(4):263-5 PMID: 9095192
  12. Immunogold evidence suggests that coupling of K+ siphoning and water transport in rat retinal Müller cells is mediated by a coenrichment of Kir4.1 and AQP4 in specific membrane domains.
    Glia. 1999 Mar;26(1):47-54 PMID: 10088671
  13. Rapid gating and anion permeability of an intracellular aquaporin.
    Nature. 1999 Nov 11;402(6758):184-7 PMID: 10647010
  14. Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein.
    Science. 1992 Apr 17;256(5055):385-7 PMID: 1373524
  15. Requirement of human renal water channel aquaporin-2 for vasopressin-dependent concentration of urine.
    Science. 1994 Apr 1;264(5155):92-5 PMID: 8140421
  16. Vasopressin increases water permeability of kidney collecting duct by inducing translocation of aquaporin-CD water channels to plasma membrane.
    Proc Natl Acad Sci U S A. 1995 Feb 14;92(4):1013-7 PMID: 7532304
  17. Projection structure of a plant vacuole membrane aquaporin by electron cryo-crystallography.
    J Mol Biol. 1999 Dec 17;294(5):1337-49 PMID: 10600389
  18. Functional reconstitution and characterization of AqpZ, the E. coli water channel protein.
    J Mol Biol. 1999 Sep 3;291(5):1169-79 PMID: 10518952
  19. Aquaporin-6: An intracellular vesicle water channel protein in renal epithelia.
    Proc Natl Acad Sci U S A. 1999 May 11;96(10):5808-13 PMID: 10318966
  20. Tetrameric assembly of CHIP28 water channels in liposomes and cell membranes: a freeze-fracture study.
    J Cell Biol. 1993 Nov;123(3):605-18 PMID: 7693713
  21. The major intrinsic protein (MIP) of the bovine lens fiber membrane: characterization and structure based on cDNA cloning.
    Cell. 1984 Nov;39(1):49-59 PMID: 6207938
  22. Aquaporins: Phylogeny, Structure, and Physiology of Water Channels.
    News Physiol Sci. 1999 Oct;14:187-193 PMID: 11390849
  23. High resolution AFM topographs of the Escherichia coli water channel aquaporin Z.
    EMBO J. 1999 Sep 15;18(18):4981-7 PMID: 10487750
  24. Phosphorylation regulates the water channel activity of the seed-specific aquaporin alpha-TIP.
    EMBO J. 1995 Jul 3;14(13):3028-35 PMID: 7542585
  25. Prediction of functional residues in water channels and related proteins.
    Protein Sci. 1998 Jun;7(6):1458-68 PMID: 9655351
  26. Inhibition of water and solute permeability in human red cells.
    Biochim Biophys Acta. 1970 Jul 7;211(1):104-6 PMID: 5470384
  27. Structural clues in the sequences of the aquaporins.
    J Mol Biol. 2000 Jan 28;295(4):1039-53 PMID: 10656809
  28. Projection structure of the CHIP28 water channel in lipid bilayer membranes at 12-A resolution.
    Biochemistry. 1994 Nov 1;33(43):12735-40 PMID: 7524655
  29. Functional unit of 30 kDa for proximal tubule water channels as revealed by radiation inactivation.
    J Biol Chem. 1991 Sep 5;266(25):16633-5 PMID: 1885592
  30. Entrance of water into human red cells under an osmotic pressure gradient.
    J Gen Physiol. 1957 Nov 20;41(2):243-57 PMID: 13475689
  31. Mutations in the founder of the MIP gene family underlie cataract development in the mouse.
    Nat Genet. 1996 Feb;12(2):212-5 PMID: 8563764
  32. Erythrocyte Mr 28,000 transmembrane protein exists as a multisubunit oligomer similar to channel proteins.
    J Biol Chem. 1991 Apr 5;266(10):6407-15 PMID: 2007592
  33. MRC image processing programs.
    J Struct Biol. 1996 Jan-Feb;116(1):9-16 PMID: 8742717
  34. Cloning and expression of apical membrane water channel of rat kidney collecting tubule.
    Nature. 1993 Feb 11;361(6412):549-52 PMID: 8429910
  35. Secretin induces the apical insertion of aquaporin-1 water channels in rat cholangiocytes.
    Am J Physiol. 1999 Jan;276(1 Pt 1):G280-6 PMID: 9887005
  36. Surface topographies at subnanometer-resolution reveal asymmetry and sidedness of aquaporin-1.
    J Mol Biol. 1996 Dec 20;264(5):907-18 PMID: 9000620
  37. Membrane topology of aquaporin CHIP. Analysis of functional epitope-scanning mutants by vectorial proteolysis.
    J Biol Chem. 1994 Jan 21;269(3):1668-73 PMID: 7507481
  38. Transport of water and glycerol in aquaporin 3 is gated by H(+).
    J Biol Chem. 1999 Jul 30;274(31):21631-6 PMID: 10419471
  39. Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons: member of an ancient channel family.
    Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11110-4 PMID: 1722319
  40. Water transport activity of the plasma membrane aquaporin PM28A is regulated by phosphorylation.
    Plant Cell. 1998 Mar;10(3):451-9 PMID: 9501117
  41. Phylogenetic characterization of the MIP family of transmembrane channel proteins.
    J Membr Biol. 1996 Oct;153(3):171-80 PMID: 8849412
  42. Functional independence of monomeric CHIP28 water channels revealed by expression of wild-type mutant heterodimers.
    J Biol Chem. 1994 Apr 8;269(14):10417-22 PMID: 7511600
  43. Lens gap junctions and orthogonal arrays are unrelated.
    FEBS Lett. 1980 Feb 25;111(1):73-8 PMID: 7358167
  44. Water transport mechanisms: water movement through lipid bilayers, pores, and plasma membranes.
    Science. 1988 Apr 8;240(4849):228 PMID: 17800923
  45. Three-dimensional organization of a human water channel.
    Nature. 1997 Jun 5;387(6633):627-30 PMID: 9177354
  46. Effect of PCMBS on CO2 permeability of Xenopus oocytes expressing aquaporin 1 or its C189S mutant.
    Am J Physiol. 1998 Dec;275(6 Pt 1):C1481-6 PMID: 9843709
  47. Regulation of aquaporin-4 water channels by phorbol ester-dependent protein phosphorylation.
    J Biol Chem. 1998 Mar 13;273(11):6001-4 PMID: 9497312
  48. Direct immunogold labeling of aquaporin-4 in square arrays of astrocyte and ependymocyte plasma membranes in rat brain and spinal cord.
    Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11981-6 PMID: 9751776
  49. Structure and dynamics of a proton wire: a theoretical study of H+ translocation along the single-file water chain in the gramicidin A channel.
    Biophys J. 1996 Jul;71(1):19-39 PMID: 8804586
  50. Structure of the osmo-regulated H2O-channel, AQP-CHIP, in projection at 3.5 A resolution.
    J Mol Biol. 1995 Aug 18;251(3):413-20 PMID: 7544415
  51. Long-term regulation of aquaporins in the kidney.
    Am J Physiol. 1999 Mar;276(3 Pt 2):F331-9 PMID: 10070156
  52. Biologically active two-dimensional crystals of aquaporin CHIP.
    J Biol Chem. 1994 Jan 21;269(3):1583-6 PMID: 8294400
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.5.800
Email
[email protected]
Published
2000-03-01
Pages
800-6
Language
English
Region
England
NLM ID
8208664
PMCID
PMC305620
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/19.5.800 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2