Home LiteratureArticle Details
PMID: 10698929 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The luminal part of the murine cytomegalovirus glycoprotein gp40 catalyzes the retention of MHC class I molecules.

The EMBO journal ·Vol. 19 ·No. 5 ·2000-03-01 ·Pages 870-81

Ziegler H, Muranyi W, Burgert HG, Kremmer E, Koszinowski UH

Abstract

Murine cytomegalovirus (MCMV) interferes with the MHC class I pathway of antigen presentation. The type I transmembrane glycoprotein gp40, encoded by the gene m152, retains major histocompatibility complex (MHC) class I complexes in the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC)/cis-Golgi. These MHC class I complexes are stable, show an extended half-life and do not exchange beta(2)-microglobulin, whereas gp40 reaches an endosomal/lysosomal compartment where it subsequently is degraded. The analysis of regions within the viral protein that are essential for MHC class I retention revealed that a secreted form of gp40, lacking the cytoplasmic tail and the transmembrane region, still has the capacity to retain MHC class I complexes. Continuous expression of gp40 is not required for MHC class I retention. Our data indicate that the retention of MHC class I complexes in the ERGIC/cis-Golgi is triggered by gp40 and does not require the further presence of the viral protein.

MeSH Terms
3T3 Cells Animals Antigen Presentation Herpesviridae Infections/immunology Major Histocompatibility Complex/immunology Membrane Glycoproteins/chemistry,genetics,immunology Mice Muromegalovirus/immunology Mutation Retroviridae Proteins/chemistry,genetics,immunology
Chemicals
Membrane Glycoproteins Retroviridae Proteins SIV envelope protein gp41
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ziegler H
Max-von-Pettenkofer Institut, Genzentrum, Ludwig-Maximilians-Universität München, Germany.
Muranyi W
Burgert H G
Kremmer E
Koszinowski U H
References (47)
47 references, click to expand
  1. A cytomegalovirus glycoprotein re-routes MHC class I complexes to lysosomes for degradation.
    EMBO J. 1999 Feb 15;18(4):1081-91 PMID: 10022849
  2. Immune evasion by cytomegalovirus--survival strategies of a highly adapted opportunist.
    Trends Microbiol. 1998 May;6(5):190-7 PMID: 9614343
  3. Characterization of plasma membrane proteins identified by monoclonal antibodies.
    J Biol Chem. 1981 Jan 25;256(2):664-71 PMID: 7451466
  4. An adenovirus glycoprotein binds heavy chains of class I transplantation antigens from man and mouse.
    Nature. 1982 Sep 9;299(5879):175-8 PMID: 6287288
  5. Inhibitors of lysosomal function.
    Methods Enzymol. 1983;96:737-64 PMID: 6361463
  6. An adenovirus type 2 glycoprotein blocks cell surface expression of human histocompatibility class I antigens.
    Cell. 1985 Jul;41(3):987-97 PMID: 3924414
  7. Structural and functional dissection of an MHC class I antigen-binding adenovirus glycoprotein.
    EMBO J. 1986 Aug;5(8):1921-7 PMID: 3019670
  8. Identification of the human cytomegalovirus glycoprotein B gene and induction of neutralizing antibodies via its expression in recombinant vaccinia virus.
    EMBO J. 1986 Nov;5(11):3057-63 PMID: 3024973
  9. Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system.
    Proc Natl Acad Sci U S A. 1987 Dec;84(23):8573-7 PMID: 2825196
  10. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
    Gene. 1988 Jul 15;67(1):31-40 PMID: 3047011
  11. Studies on the mechanisms of autophagy: maturation of the autophagic vacuole.
    J Cell Biol. 1990 Jun;110(6):1935-45 PMID: 2161853
  12. Identification of a consensus motif for retention of transmembrane proteins in the endoplasmic reticulum.
    EMBO J. 1990 Oct;9(10):3153-62 PMID: 2120038
  13. Novel purification of the catalytic domain of Golgi alpha-mannosidase II. Characterization and comparison with the intact enzyme.
    J Biol Chem. 1991 Sep 5;266(25):16876-85 PMID: 1885615
  14. Efficient processing of an antigenic sequence for presentation by MHC class I molecules depends on its neighboring residues in the protein.
    Cell. 1991 Sep 20;66(6):1145-53 PMID: 1913805
  15. Russell bodies: a general response of secretory cells to synthesis of a mutant immunoglobulin which can neither exit from, nor be degraded in, the endoplasmic reticulum.
    J Cell Biol. 1991 Nov;115(4):983-94 PMID: 1955467
  16. Distribution of the intermediate elements operating in ER to Golgi transport.
    J Cell Sci. 1991 Nov;100 ( Pt 3):415-30 PMID: 1808196
  17. Cytomegalovirus prevents antigen presentation by blocking the transport of peptide-loaded major histocompatibility complex class I molecules into the medial-Golgi compartment.
    J Exp Med. 1992 Sep 1;176(3):729-38 PMID: 1324970
  18. The motif Tyr-X-X-hydrophobic residue mediates lysosomal membrane targeting of lysosome-associated membrane protein 1.
    J Biol Chem. 1993 Jan 25;268(3):1941-6 PMID: 8420968
  19. Virus strategies for evasion of the host response to infection.
    Trends Microbiol. 1994 Mar;2(3):81-8 PMID: 7512419
  20. pH-independent and -dependent cleavage of proinsulin in the same secretory vesicle.
    J Cell Biol. 1994 Sep;126(5):1149-56 PMID: 8063854
  21. Herpes simplex virus turns off the TAP to evade host immunity.
    Nature. 1995 Jun 1;375(6530):411-5 PMID: 7760935
  22. A viral inhibitor of peptide transporters for antigen presentation.
    Nature. 1995 Jun 1;375(6530):415-8 PMID: 7760936
  23. Construction of a novel bovine papillomavirus vector without detectable transforming activity suitable for gene transfer.
    Hum Gene Ther. 1995 Mar;6(3):325-33 PMID: 7779916
  24. Identification of the mouse cytomegalovirus genomic region affecting major histocompatibility complex class I molecule transport.
    J Virol. 1995 Oct;69(10):6098-105 PMID: 7666513
  25. Quality control in the secretory pathway.
    Curr Opin Cell Biol. 1995 Aug;7(4):523-9 PMID: 7495572
  26. Protein sorting by transport vesicles.
    Science. 1996 Apr 12;272(5259):227-34 PMID: 8602507
  27. The human cytomegalovirus US11 gene product dislocates MHC class I heavy chains from the endoplasmic reticulum to the cytosol.
    Cell. 1996 Mar 8;84(5):769-79 PMID: 8625414
  28. Molecular mechanism and species specificity of TAP inhibition by herpes simplex virus ICP47.
    EMBO J. 1996 Jul 1;15(13):3247-55 PMID: 8670825
  29. Bimodal interaction of coatomer with the p24 family of putative cargo receptors.
    Science. 1996 Sep 6;273(5280):1396-9 PMID: 8703076
  30. Human cytomegalovirus inhibits antigen presentation by a sequential multistep process.
    Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10990-5 PMID: 8855296
  31. Subversion of the MHC class I antigen-presentation pathway by adenoviruses and herpes simplex viruses.
    Trends Microbiol. 1996 Mar;4(3):107-12 PMID: 8868089
  32. Human cytomegalovirus US3 impairs transport and maturation of major histocompatibility complex class I heavy chains.
    Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11327-33 PMID: 8876135
  33. Sec61-mediated transfer of a membrane protein from the endoplasmic reticulum to the proteasome for destruction.
    Nature. 1996 Dec 5;384(6608):432-8 PMID: 8945469
  34. Analysis of the complete DNA sequence of murine cytomegalovirus.
    J Virol. 1996 Dec;70(12):8833-49 PMID: 8971012
  35. Separation of PP2A core enzyme and holoenzyme with monoclonal antibodies against the regulatory A subunit: abundant expression of both forms in cells.
    Mol Cell Biol. 1997 Mar;17(3):1692-701 PMID: 9032296
  36. A mouse cytomegalovirus glycoprotein, gp34, forms a complex with folded class I MHC molecules in the ER which is not retained but is transported to the cell surface.
    EMBO J. 1997 Feb 17;16(4):685-94 PMID: 9049298
  37. Misfolded major histocompatibility complex class I heavy chains are translocated into the cytoplasm and degraded by the proteasome.
    Proc Natl Acad Sci U S A. 1997 Mar 4;94(5):1896-901 PMID: 9050876
  38. A mouse cytomegalovirus glycoprotein retains MHC class I complexes in the ERGIC/cis-Golgi compartments.
    Immunity. 1997 Jan;6(1):57-66 PMID: 9052837
  39. Dissociation of coatomer from membranes is required for brefeldin A-induced transfer of Golgi enzymes to the endoplasmic reticulum.
    J Cell Biol. 1997 Apr 21;137(2):319-33 PMID: 9128245
  40. Transmembrane domain-dependent sorting of proteins to the ER and plasma membrane in yeast.
    EMBO J. 1997 Apr 15;16(8):1832-41 PMID: 9155009
  41. The ER-luminal domain of the HCMV glycoprotein US6 inhibits peptide translocation by TAP.
    Immunity. 1997 May;6(5):613-21 PMID: 9175839
  42. A viral ER-resident glycoprotein inactivates the MHC-encoded peptide transporter.
    Immunity. 1997 May;6(5):623-32 PMID: 9175840
  43. The human cytomegalovirus US6 glycoprotein inhibits transporter associated with antigen processing-dependent peptide translocation.
    Proc Natl Acad Sci U S A. 1997 Jun 24;94(13):6904-9 PMID: 9192664
  44. Interference with antigen processing by viruses.
    Curr Opin Immunol. 1997 Aug;9(4):470-6 PMID: 9287180
  45. Herpesvirus evasion of the immune system.
    Curr Top Microbiol Immunol. 1998;232:149-77 PMID: 9557398
  46. Mechanisms of MHC class I--restricted antigen processing.
    Annu Rev Immunol. 1998;16:323-58 PMID: 9597133
  47. Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169.
    Curr Top Microbiol Immunol. 1990;154:125-69 PMID: 2161319
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.5.870
Published
2000-03-01
Pages
870-81
Language
English
Region
England
NLM ID
8208664
PMCID
PMC305627
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/19.5.870 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2