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PMID: 10698950 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Charged tmRNA but not tmRNA-mediated proteolysis is essential for Neisseria gonorrhoeae viability.

The EMBO journal ·Vol. 19 ·No. 5 ·2000-03-01 ·Pages 1098-107

Huang C, Wolfgang MC, Withey J, Koomey M, Friedman DI

Abstract

tmRNA, through its tRNA and mRNA properties, adds short peptide tags to abnormal proteins, targeting these proteins for proteolytic degradation. Although the conservation of tmRNA throughout the bacterial kingdom suggests that it must provide a strong selective advantage, it has not been shown to be essential for any bacterium. We report that tmRNA is essential in Neisseria gonorrhoeae. Although tagging per se appears to be required for gonococcal viability, tagging for proteolysis does not. This suggests that the essential roles of tmRNA in N.gonorrhoeae may include resolving stalled translation complexes and/or preventing depletion of free ribosomes. Although derivatives of N.gonorrhoeae expressing Escherichia coli tmRNA as their sole tmRNA were isolated, they appear to form colonies only after acquiring an extragenic suppressor(s).

MeSH Terms
Base Sequence Molecular Sequence Data Neisseria gonorrhoeae/physiology Protein Biosynthesis RNA, Bacterial/physiology RNA, Messenger/physiology RNA, Transfer/physiology
Chemicals
RNA, Bacterial RNA, Messenger tmRNA RNA, Transfer
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Huang C
Graduate Program in Cellular and Molecular Biology, Medical School, The University of Michigan, Ann Arbor, MI 48109, USA.
Wolfgang M C
Withey J
Koomey M
Friedman D I
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.5.1098
Published
2000-03-01
Pages
1098-107
Language
English
Region
England
NLM ID
8208664
PMCID
PMC305648
Subset
IM
Grants
NCRR NIH HHS · MO1-RR0042 · United States
NIAID NIH HHS · AI11459-10 · United States
NCRR NIH HHS · M01 RR000042 · United States
NIAID NIH HHS · AI27837 · United States
NIGMS NIH HHS · T32 GM007315 · United States
External Links
PubMed source DOI: 10.1093/emboj/19.5.1098 PMC Full Text View journal details
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