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PMID: 1070013 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Single burst study of rec- and red-mediated recombination in bacteriophage lambda.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 12 ·1976-12-00 ·Pages 4613-7

Sarthy PV, Meselson M

Abstract

Single bursts from three-point crosses of bacteriophage lambda were analyzed for recombination of markers several thousand nucleotide pairs apart. Single recombination, mediated by the rec system of Escherichia coli, is usually reciprocal. Double recombinants are also significantly correlated in single bursts, although the correlation is weaker than for reciprocal singles. Reciprocity is not found in crosses mediated by the lambda red system. With respect to certain other paraments of recombination, the two systems appear to be alike. Double recombinants usually result from one event, not from two independent single recombinations, and their average clone size is about half that of single recombinants. The results are discussed in terms of current molecular models of recombination.

MeSH Terms
Base Sequence Chromosome Mapping Coliphages/physiology Genes Genes, Regulator Recombination, Genetic
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sarthy P V
Meselson M
References (23)
23 references, click to expand
  1. Localized Negative Interference in Bacteriophage.
    Genetics. 1965 Mar;51(3):369-79 PMID: 17248239
  2. Genetic Recombination between Host-Range and Plaque-Type Mutants of Bacteriophage in Single Bacterial Cells.
    Genetics. 1949 Jan;34(1):44-71 PMID: 17247304
  3. The effects of the decay of incorporated radioactive phosphorus on the genome of bacteriophage T4.
    Virology. 1956 Apr;2(2):206-34 PMID: 13312222
  4. A genetic study of the temperate coliphage.
    Virology. 1955 Nov;1(4):424-43 PMID: 13274744
  5. The production of phage chromosome fragments and their capacity for genetic transfer.
    J Mol Biol. 1962 Apr;4:275-87 PMID: 14453145
  6. Genetic study of a temperate bacteriophage of Escherichia Coli. II. Mechanism of genetic recombination.
    Ann Inst Pasteur (Paris). 1954 Dec;87(6):674-90 PMID: 14350340
  7. The frequency distribution of spontaneous bacteriophage mutants as evidence for the exponential rate of phage reproduction.
    Cold Spring Harb Symp Quant Biol. 1951;16:463-70 PMID: 14942756
  8. Molecular aspects of genetic exchange and gene conversion.
    Genetics. 1974 Sep;78(1):273-87 PMID: 4613609
  9. Molecular basis for genetic recombination.
    Genetics. 1974 Sep;78(1):247-57 PMID: 4613607
  10. Symposium No. 5: Genetic exchange. Introduction by the Chairman. Advances in recombination research.
    Genetics. 1974 Sep;78(1):237-45 PMID: 4613606
  11. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
  12. A reanalysis of red-mediated reciprocal recombination in bacteriophage lambda.
    Virology. 1972 Aug;49(2):599-601 PMID: 4559691
  13. On the physical structure of lambda recombinant DNA.
    Mol Gen Genet. 1973 May 28;122(4):353-66 PMID: 4716874
  14. Branched DNA molecules: intermediates in T4 recombination.
    J Mol Biol. 1971 Aug 28;60(1):131-49 PMID: 4937189
  15. Identification of recombinant chromosomes and F-merogenotes in merodiploids of Escherichia coli.
    J Bacteriol. 1968 Jul;96(1):173-9 PMID: 4874304
  16. New mutations in the S cistron of bacteriophage lambda affecting host cell lysis.
    Virology. 1969 May;38(1):200-2 PMID: 4891223
  17. Reciprocal recombination of chromosome and F. merogenote in Escherichia coli.
    J Bacteriol. 1965 Dec;90(6):1664-8 PMID: 5322723
  18. Isolation and characterization of lambda transducing bacteriophages for argF, argI and adjacent genes.
    J Bacteriol. 1975 May;122(2):727-42 PMID: 1092666
  19. Mismatch repair in heteroduplex DNA.
    Proc Natl Acad Sci U S A. 1975 Jun;72(6):2202-6 PMID: 1094458
  20. A general model for genetic recombination.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):358-61 PMID: 1054510
  21. Reciprocal and non-reciprocal recombination in bacteriopahge lambda.
    J Mol Biol. 1969 Jul 28;43(2):351-5 PMID: 5804921
  22. Recombination in bacteriophage lambda. I. Mutants deficient in general recombination.
    J Mol Biol. 1968 Jul 14;34(2):261-71 PMID: 5760458
  23. Reciprocal recombination in prophage lambda.
    J Cell Physiol. 1967 Oct;70(2):Suppl:113-8 PMID: 5582803
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.12.4613
Published
1976-12-00
Pages
4613-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431566
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.12.4613 PMC Full Text View journal details
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