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PMID: 10712526 Published · ppublish English Journal Article

Purification of a jojoba embryo fatty acyl-coenzyme A reductase and expression of its cDNA in high erucic acid rapeseed.

Plant physiology ·Vol. 122 ·No. 3 ·2000-03-00 ·Pages 635-44

Metz JG, Pollard MR, Anderson L, Hayes TR, Lassner MW

Abstract

The jojoba (Simmondsia chinensis) plant produces esters of long-chain alcohols and fatty acids (waxes) as a seed lipid energy reserve. This is in contrast to the triglycerides found in seeds of other plants. We purified an alcohol-forming fatty acyl-coenzyme A reductase (FAR) from developing embryos and cloned the cDNA encoding the enzyme. Expression of a cDNA in Escherichia coli confers FAR activity upon those cells and results in the accumulation of fatty alcohols. The FAR sequence shows significant homology to an Arabidopsis protein of unknown function that is essential for pollen development. When the jojoba FAR cDNA is expressed in embryos of Brassica napus, long-chain alcohols can be detected in transmethylated seed oils. Resynthesis of the gene to reduce its A plus T content resulted in increased levels of alcohol production. In addition to free alcohols, novel wax esters were detected in the transgenic seed oils. In vitro assays revealed that B. napus embryos have an endogenous fatty acyl-coenzyme A: fatty alcohol acyl-transferase activity that could account for this wax synthesis. Thus, introduction of a single cDNA into B. napus results in a redirection of a portion of seed oil synthesis from triglycerides to waxes.

MeSH Terms
Aldehyde Oxidoreductases/genetics,isolation & purification Amino Acid Sequence Base Sequence Brassica/genetics,metabolism Cloning, Molecular DNA Primers/genetics DNA, Complementary/genetics DNA, Plant/genetics Erucic Acids/metabolism Escherichia coli/genetics Gene Expression Molecular Sequence Data Plant Oils/metabolism Plants/embryology,enzymology,genetics Plants, Genetically Modified
Chemicals
DNA Primers DNA, Complementary DNA, Plant Erucic Acids Plant Oils erucic acid Aldehyde Oxidoreductases hexadecanal dehydrogenase (acylating)
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Metz J G
Calgene Campus, Monsanto, 1920 Fifth Street, Davis, California 95616, USA. [email protected]
Pollard M R
Anderson L
Hayes T R
Lassner M W
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
Published
2000-03-00
Pages
635-44
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC58898
Subset
IM
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