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PMID: 10716703 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Photophysics and optical switching in green fluorescent protein mutants.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 97 ·No. 7 ·2000-03-28 ·Pages 2974-8

Creemers TM, Lock AJ, Subramaniam V, Jovin TM, Völker S

Abstract

We demonstrate by using low-temperature high-resolution spectroscopy that red-shifted mutants of green fluorescent protein are photo-interconverted among three conformations and are, therefore, not photostable "one-color" systems as previously believed. From our experiments we have further derived the energy-level schemes governing the interconversion among the three forms. These results have significant implications for the molecular and cell biological applications of the green fluorescent protein family; for example, in fluorescence resonant energy transfer experiments, a change in "color" on irradiation may not necessarily be due to energy transfer but can also arise from a photo-induced conversion between conformers of the excited species.

MeSH Terms
Green Fluorescent Proteins Luminescent Proteins/chemistry,genetics Mutagenesis Photochemistry Spectrometry, Fluorescence Spectrophotometry, Ultraviolet
Chemicals
Luminescent Proteins Green Fluorescent Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Creemers T M
Center for the Study of Excited States of Molecules, Huygens and Gorlaeus Laboratories, University of Leiden, P.O. Box 9504, 2300 RA Leiden, The Netherlands.
Lock A J
Subramaniam V
Jovin T M
Völker S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.050365997
Published
2000-03-28
Pages
2974-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC16176
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.050365997 PMC Full Text View journal details
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