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PMID: 10725374 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Status epilepticus decreases glutamate receptor 2 mRNA and protein expression in hippocampal pyramidal cells before neuronal death.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 97 ·No. 7 ·2000-03-28 ·Pages 3631-6

Grooms SY, Opitz T, Bennett MV, Zukin RS

Abstract

Kainic acid (KA)-induced status epilepticus in adult rats leads to delayed, selective death of pyramidal neurons in the hippocampal CA1 and CA3. Death is preceded by down-regulation of glutamate receptor 2 (GluR2) mRNA and protein [the subunit that limits Ca(2+) permeability of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors] in CA1 and CA3, as indicated by in situ hybridization, immunolabeling, and quantitative Western blotting. GluR1 mRNA and protein are unchanged or slightly increased before cell death. These changes could lead to formation of GluR2-lacking, Ca(2+)-permeable AMPA receptors and increased toxicity of endogenous glutamate. GluR2 immunolabeling is unchanged in granule cells of the dentate gyrus, which are resistant to seizure-induced death. Thus, formation of Ca(2+)-permeable AMPA receptors may be a critical mediator of delayed neurodegeneration after status epilepticus.

MeSH Terms
Animals Behavior, Animal Blotting, Western Cell Death/genetics Down-Regulation Hippocampus/cytology,metabolism Immunohistochemistry Male Neurons/cytology,metabolism RNA, Messenger/genetics,metabolism Rats Rats, Sprague-Dawley Receptors, Glutamate/genetics,metabolism Seizures/genetics,metabolism Status Epilepticus/genetics
Chemicals
RNA, Messenger Receptors, Glutamate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Grooms S Y
Department of Neuroscience, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
Opitz T
Bennett M V
Zukin R S
References (29)
29 references, click to expand
  1. Differential dependence on GluR2 expression of three characteristic features of AMPA receptors.
    J Neurosci. 1997 Dec 15;17(24):9393-406 PMID: 9390995
  2. The GluR2 (GluR-B) hypothesis: Ca(2+)-permeable AMPA receptors in neurological disorders.
    Trends Neurosci. 1997 Oct;20(10):464-70 PMID: 9347614
  3. Zinc and brain injury.
    Annu Rev Neurosci. 1998;21:347-75 PMID: 9530500
  4. Kainate receptors: an interplay between excitatory and inhibitory synapses.
    FEBS Lett. 1998 Jun 23;430(1-2):100-4 PMID: 9678603
  5. Interaction of the N-ethylmaleimide-sensitive factor with AMPA receptors.
    Neuron. 1998 Aug;21(2):393-400 PMID: 9728920
  6. Selective reduction of GluR2 protein in adult hippocampal CA3 neurons following status epilepticus but prior to cell loss.
    Hippocampus. 1998;8(5):511-25 PMID: 9825961
  7. Turnover analysis of glutamate receptors identifies a rapidly degraded pool of the N-methyl-D-aspartate receptor subunit, NR1, in cultured cerebellar granule cells.
    J Biol Chem. 1999 Jan 1;274(1):151-7 PMID: 9867823
  8. GluR5 kainate receptor activation in interneurons increases tonic inhibition of pyramidal cells.
    Nat Neurosci. 1998 Oct;1(6):470-8 PMID: 10196544
  9. Synaptic activation of kainate receptors on hippocampal interneurons.
    Nat Neurosci. 1998 Oct;1(6):479-86 PMID: 10196545
  10. Surface expression of AMPA receptors in hippocampal neurons is regulated by an NSF-dependent mechanism.
    Neuron. 1999 Jun;23(2):365-76 PMID: 10399941
  11. Knockdown of AMPA receptor GluR2 expression causes delayed neurodegeneration and increases damage by sublethal ischemia in hippocampal CA1 and CA3 neurons.
    J Neurosci. 1999 Nov 1;19(21):9218-27 PMID: 10531425
  12. Widespread patterns of neuronal damage following systemic or intracerebral injections of kainic acid: a histological study.
    Neuroscience. 1980;5(6):991-1014 PMID: 7402461
  13. Minireview. Kainic acid as a tool for the study of temporal lobe epilepsy.
    Life Sci. 1981 Nov 16;29(20):2031-42 PMID: 7031398
  14. Limbic seizure and brain damage produced by kainic acid: mechanisms and relevance to human temporal lobe epilepsy.
    Neuroscience. 1985 Feb;14(2):375-403 PMID: 2859548
  15. Differential expression of three glutamate receptor genes in developing rat brain: an in situ hybridization study.
    Proc Natl Acad Sci U S A. 1991 May 15;88(10):4157-61 PMID: 1851996
  16. The characterization and localization of the glutamate receptor subunit GluR1 in the rat brain.
    J Neurosci. 1991 Sep;11(9):2713-24 PMID: 1652625
  17. Biochemical characterization and localization of a non-N-methyl-D-aspartate glutamate receptor in rat brain.
    J Neurochem. 1992 Mar;58(3):1118-26 PMID: 1371146
  18. The striatal mosaic in primates: striosomes and matrix are differentially enriched in ionotropic glutamate receptor subunits.
    J Neurosci. 1993 Feb;13(2):782-92 PMID: 7678861
  19. AMPA glutamate receptor subunits are differentially distributed in rat brain.
    Neuroscience. 1993 Mar;53(2):327-58 PMID: 8388083
  20. Alterations of the GluR-B AMPA receptor subunit flip/flop expression in kainate-induced epilepsy and ischemia.
    Neuroscience. 1993 Dec;57(3):545-54 PMID: 8309523
  21. Kainate-induced status epilepticus alters glutamate and GABAA receptor gene expression in adult rat hippocampus: an in situ hybridization study.
    J Neurosci. 1994 May;14(5 Pt 1):2697-707 PMID: 8182436
  22. The role of zinc in selective neuronal death after transient global cerebral ischemia.
    Science. 1996 May 17;272(5264):1013-6 PMID: 8638123
  23. An immunocytochemical study of glutamate receptors and glutamine synthetase in the hippocampus of rats injected with kainate.
    Exp Brain Res. 1996 May;109(2):251-67 PMID: 8738374
  24. Differential regulation of NMDAR1 mRNA and protein by estradiol in the rat hippocampus.
    J Neurosci. 1996 Nov 1;16(21):6830-8 PMID: 8824322
  25. Synaptic distribution of the AMPA-GluR2 subunit and its colocalization with calcium-binding proteins in rat cerebral cortex: an immunohistochemical study using a GluR2-specific monoclonal antibody.
    Exp Neurol. 1996 Dec;142(2):296-312 PMID: 8934561
  26. Seizure-related changes in the glutamate R2 and R5 receptor genes expression in the rat hippocampal formation.
    J Neural Transm (Vienna). 1997;104(2-3):125-33 PMID: 9203076
  27. Global ischemia induces downregulation of Glur2 mRNA and increases AMPA receptor-mediated Ca2+ influx in hippocampal CA1 neurons of gerbil.
    J Neurosci. 1997 Aug 15;17(16):6179-88 PMID: 9236229
  28. The GluR2 hypothesis: Ca(++)-permeable AMPA receptors in delayed neurodegeneration.
    Cold Spring Harb Symp Quant Biol. 1996;61:373-84 PMID: 9246466
  29. Kainate-stimulated Zn2+ uptake labels cortical neurons with Ca2+-permeable AMPA/kainate channels.
    Brain Res. 1998 Jan 19;781(1-2):45-56 PMID: 9507061
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.050586497
Published
2000-03-28
Pages
3631-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC16291
Subset
IM
Grants
NINDS NIH HHS · R01 NS020752 · United States
NINDS NIH HHS · NS 20752 · United States
NINDS NIH HHS · NS 31282 · United States
External Links
PubMed source DOI: 10.1073/pnas.050586497 PMC Full Text View journal details
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