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PMID: 10727431 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Properties of a polyamine transporter regulated by antizyme.

The Biochemical journal ·Vol. 347 Pt 1 ·2000-04-01 ·Pages 297-303

Sakata K, Kashiwagi K, Igarashi K

Abstract

The regulation of polyamine transport by antizyme, a protein that is involved in the rapid degradation of ornithine decarboxylase (ODC), was studied in FM3A mouse cells overproducing ODC. Both artificial (Z1) and natural antizymes not only inhibited polyamine uptake but also stimulated polyamine excretion. The properties of the polyamine transporter regulated by antizyme were characterized. The uptake of radiolabelled polyamines was inhibited by excess acetylpolyamines and a protonophore, CCCP (carbonyl cyanide m-chlorophenylhydrazone), whereas the excretion of radiolabelled polyamines was stimulated by unlabelled polyamines, acetylpolyamines and CCCP in the medium. Furthermore, it is shown that polyamines and acetylpolyamines are excreted from cells. On the basis of the results, it is discussed how antizyme regulates polyamine transport negatively.

MeSH Terms
Animals Biological Transport/drug effects Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology Carrier Proteins/metabolism Cell Line Indicators and Reagents Kinetics Mice Onium Compounds/pharmacokinetics Organophosphorus Compounds/pharmacokinetics Ornithine Decarboxylase/metabolism Polyamines/metabolism Proteins/metabolism Putrescine/metabolism Recombinant Proteins/metabolism Spermidine/metabolism Spermine/metabolism Transfection
Chemicals
Carrier Proteins Indicators and Reagents Onium Compounds Organophosphorus Compounds Polyamines Proteins Recombinant Proteins ornithine decarboxylase antizyme Spermine Carbonyl Cyanide m-Chlorophenyl Hydrazone Ornithine Decarboxylase Spermidine tetraphenylphosphonium Putrescine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sakata K
Faculty of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.
Kashiwagi K
Igarashi K
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
Published
2000-04-01
Pages
297-303
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1220960
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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