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PMID: 10747093 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evidence for the involvement of KIF4 in the anterograde transport of L1-containing vesicles.

The Journal of cell biology ·Vol. 149 ·No. 1 ·2000-04-03 ·Pages 141-52

Peretti D, Peris L, Rosso S, Quiroga S, Cáceres A

Abstract

In this study we present evidence about the cellular functions of KIF4. Using subcellular fractionation techniques and immunoisolation, we have now identified a type of vesicle that associates with KIF4, an NH(2)-terminal globular motor domain kinesin-like protein. This vesicle is highly concentrated in growth cones and contains L1, a cell adhesion molecule implicated in axonal elongation. It lacks synaptic vesicle markers, receptors for neurotrophins, and membrane proteins involved in growth cone guidance. In cultured neurons, KIF4 and L1 predominantly localize to the axonal shaft and its growth cone. Suppression of KIF4 with antisense oligonucleotides results in the accumulation of L1 within the cell body and in its complete disappearance from axonal tips. In addition, KIF4 suppression prevents L1-enhanced axonal elongation. Taken collectively, our results suggest an important role for KIF4 during neuronal development, a phenomenon which may be related to the anterograde transport of L1-containing vesicles.

MeSH Terms
Animals Antibody Specificity Axonal Transport Axons/metabolism Biomarkers/analysis Cells, Cultured Cerebral Cortex/cytology Fluorescent Antibody Technique Growth Cones/metabolism Intracellular Membranes/chemistry,metabolism Kinesins/chemistry,genetics,immunology,metabolism Leukocyte L1 Antigen Complex Membrane Glycoproteins/metabolism Mice Microsomes/chemistry,metabolism Molecular Weight Nerve Tissue Proteins Neural Cell Adhesion Molecules/metabolism Oligonucleotides, Antisense/genetics Pyramidal Cells/cytology,embryology,metabolism Rats Synaptic Vesicles/chemistry,metabolism Vacuoles/chemistry,metabolism
Chemicals
Biomarkers Leukocyte L1 Antigen Complex Membrane Glycoproteins Nerve Tissue Proteins Neural Cell Adhesion Molecules Oligonucleotides, Antisense Kif4 protein, mouse Kinesins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Peretti D
Instituto Investigación Médica Mercedes y Martín Ferreyra (INIMEC-CONICET), 5000 Córdoba, Argentina.
Peris L
Rosso S
Quiroga S
Cáceres A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.149.1.141
Published
2000-04-03
Pages
141-52
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2175094
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.149.1.141 PMC Full Text View journal details
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