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PMID: 10788368 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Redirection of the respiro-fermentative flux distribution in Saccharomyces cerevisiae by overexpression of the transcription factor Hap4p.

Applied and environmental microbiology ·Vol. 66 ·No. 5 ·2000-05-00 ·Pages 1970-3

Blom J, De Mattos MJ, Grivell LA

Abstract

Reduction of aerobic fermentation on sugars by altering the fermentative/oxidative balance is of significant interest for optimization of industrial production of Saccharomyces cerevisiae. Glucose control of oxidative metabolism in baker's yeast is partly mediated through transcriptional regulation of the Hap4p subunit of the Hap2/3/4/5p transcriptional activator complex. To alleviate glucose repression of oxidative metabolism, we constructed a yeast strain with constitutively elevated levels of Hap4p. Genetic analysis of expression levels of glucose-repressed genes and analysis of respiratory capacity showed that Hap4p overexpression (partly) relieves glucose repression of respiration. Analysis of the physiological properties of the Hap4p overproducer in batch cultures in fermentors (aerobic, glucose excess) has shown that the metabolism of this strain is more oxidative than in the wild-type strain, resulting in a significant reduced ethanol production and improvement of growth rate and a 40% gain in biomass yield. Our results show that modification of one or more transcriptional regulators can be a powerful and a widely applicable tool for redirection of metabolic fluxes in microorganisms.

MeSH Terms
CCAAT-Binding Factor Culture Media DNA-Binding Proteins/genetics,metabolism Fermentation Fungal Proteins/genetics,metabolism Gene Expression Regulation, Fungal/drug effects Glucose/metabolism,pharmacology Oxygen Consumption Saccharomyces cerevisiae/genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins Transcription Factors/genetics,metabolism Transcription, Genetic
Chemicals
CCAAT-Binding Factor Culture Media DNA-Binding Proteins Fungal Proteins HAP4 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Glucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Blom J
Section for Molecular Biology, Swammerdam Institute of Life Sciences, University of Amsterdam, 1098 SM Amsterdam, The Netherlands.
De Mattos M J
Grivell L A
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
Published
2000-05-00
Pages
1970-3
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC101441
Subset
IM
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