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PMID: 10793133 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Response of Xenopus Cds1 in cell-free extracts to DNA templates with double-stranded ends.

Molecular biology of the cell ·Vol. 11 ·No. 5 ·2000-05-00 ·Pages 1535-46

Guo Z, Dunphy WG

Abstract

Although homologues of the yeast checkpoint kinases Cds1 and Chk1 have been identified in various systems, the respective roles of these kinases in the responses to damaged and/or unreplicated DNA in vertebrates have not been delineated precisely. Likewise, it is largely unknown how damaged DNA and unreplicated DNA trigger the pathways that contain these effector kinases. We report that Xenopus Cds1 (Xcds1) is phosphorylated and activated by the presence of some simple DNA molecules with double-stranded ends in cell-free Xenopus egg extracts. Xcds1 is not affected by aphidicolin, an agent that induces DNA replication blocks. In contrast, Xenopus Chk1 (Xchk1) responds to DNA replication blocks but not to the presence of double-stranded DNA ends. Immunodepletion of Xcds1 (and/or Xchk1) from egg extracts did not attenuate the cell cycle delay induced by double-stranded DNA ends. These results imply that the cell cycle delay triggered by double-stranded DNA ends either does not involve Xcds1 or uses a factor(s) that can act redundantly with Xcds1.

MeSH Terms
Amino Acid Sequence Animals Aphidicolin/pharmacology Caffeine/pharmacology Cell-Free System Checkpoint Kinase 1 Checkpoint Kinase 2 Cloning, Molecular DNA/drug effects,genetics,metabolism DNA Damage/drug effects,radiation effects Mitosis Molecular Sequence Data Phosphorylation Poly T/metabolism Protein Kinases/genetics,immunology,metabolism Protein Serine-Threonine Kinases Sequence Homology, Amino Acid Serine/metabolism Templates, Genetic Ultraviolet Rays Xenopus Proteins ras-GRF1/metabolism
Chemicals
Xenopus Proteins ras-GRF1 Poly T Aphidicolin Caffeine Serine DNA Protein Kinases Checkpoint Kinase 2 Checkpoint Kinase 1 Chek1 protein, Xenopus Protein Serine-Threonine Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Guo Z
Division of Biology, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California 91125, USA.
Dunphy W G
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.11.5.1535
Published
2000-05-00
Pages
1535-46
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC14865
Subset
IM
Databases
GENBANK
AF174295
External Links
PubMed source DOI: 10.1091/mbc.11.5.1535 PMC Full Text View journal details
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