Unpaired bases in superhelical DNA: kinetic evidence.

Nucleic acids research ·Vol. 1 ·No. 4 ·1974-04-00 ·Pages 549-58

Jacob RJ, Lebowitz J, Printz MP

Abstract

Kinetic analysis of the early, fast reaction of superhelical DNA with formaldehyde reveals that this region or regions is 56% "single strand like" in character. Hydrogen-tritium exchange studies coupled with other considerations show that this reaction is not due to a difference in conformational motility between form I and form II molecules, but is due to unpaired or weakly hydrogen bonded, localized region(s) of the form I allomorph of circular DNA.

MeSH Terms

Base Pairing DNA, Superhelical/chemistry Hydrogen Bonding Kinetics Models, Chemical Nucleic Acid Conformation

Chemicals

DNA, Superhelical

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Jacob R J

Lebowitz J

Printz M P

References (13)

13 references, click to expand

Hydrogen exchange in proteins.
Adv Protein Chem. 1966;21:287-386 PMID: 5333290
On the kinetics of hydrogen exchange in deoxyribonucleic acid. pH and salt effects.
Biochemistry. 1968 Sep;7(9):3194-206 PMID: 5697655
Interaction of closed circular DNA with intercalative dyes. II. The free energy of superhelix formation in SV40 DNA.
J Mol Biol. 1970 Feb 14;47(3):419-35 PMID: 4313852
Tritium-hydrogen exchange studies of polynucleotides. Double-stranded polyriboadenylic acid.
Biochemistry. 1970 Jul 21;9(15):3077-87 PMID: 5474807
Hydrogen exchange as a probe of the dynamic structure of DNA. I. General acid-base catalysis.
J Mol Biol. 1970 Jun 14;50(2):297-316 PMID: 5529262
Hydrogen exchange as a probe of the dynamic structure of DNA. II. Effects of base composition and destabilizing salts.
J Mol Biol. 1970 Jun 14;50(2):317-32 PMID: 4320048
Further analysis of the altered secondary structure of superhelical DNA. Sensitivity to methylmercuric hydroxide a chemical probe for unpaired bases.
J Mol Biol. 1973 Sep 25;79(3):451-70 PMID: 4758062
Kinetic studies of denaturation and reaction with formaldehyde on polydeoxyribonucleotides.
Biochemistry. 1971 Mar 30;10(7):1254-64 PMID: 5553329
A study of rapid hydrogen exchange in nucleic acids.
J Mol Biol. 1971 Jun 28;58(3):847-63 PMID: 4326404
Dynamic aspects of native DNA structure: kinetics of the formaldehyde reaction with calf thymus DNA.
J Mol Biol. 1971 Nov 14;61(3):587-613 PMID: 5167307
Slow exchange of the "outside" amino hydrogens of DNA.
J Mol Biol. 1972 Jan 14;63(1):171-7 PMID: 5062841
Sensitivity of superhelical DNA to a single-strand specific endonuclease.
Biochim Biophys Acta. 1973 Apr 21;308(7):68-78 PMID: 4269186
Studies on the secondary structure of phenylalanyl transfer ribonucleic acid.
Biochemistry. 1970 Dec 8;9(25):4971-80 PMID: 4921070

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/1.4.549

Published

1974-04-00

Pages

549-58

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343356

Subset

External Links

PubMed source DOI: 10.1093/nar/1.4.549 PMC Full Text View journal details

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Unpaired bases in superhelical DNA: kinetic evidence.

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