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PMID: 1081384 Published · ppublish English Journal Article

Cytoplasmic and outer membranes separation in Rhodopseudomonas sphaeroides.

Archives of microbiology ·Vol. 105 ·No. 3 ·1975-11-07 ·Pages 269-75

Guillotin J, Reiss-Husson F

Abstract

A cell envelope fraction had been prepared after mechanical disruption of lysozyme-EDTA spheroplasts from depigmented Rhodopseudomonas sphaeroides (aerobically grown in the light). On linear sucrose gradients this fraction can be separated in a cytoplasmic membrane fraction and an outer membrane fraction. The cytoplasmic fraction (buoyant density: 1.18 g/cm3) has been characterized by its succinic dehydrogenase activity and by its composition. The outer membrane fraction (buoyant density: 1.21 g/cm3) does not contain any respiratory activity nor hemoproteins. The same fractionation has been done on cells repigmented in the dark by lowering the O2 pressure. In that case the same two fractions have been detected in addition to the chromatophore fraction (buoyant density: 1.14 g/cm3). However both, and specially the outer membrane fraction, were contaminated by chromatophore material.

MeSH Terms
Aerobiosis Bacterial Chromatophores Bacteriochlorophylls/analysis Carotenoids/analysis Cell Fractionation/methods Cell Wall/analysis Centrifugation, Density Gradient Darkness Hemeproteins/analysis Membranes/analysis Oxygen Partial Pressure Photosynthesis Rhodobacter sphaeroides/cytology Spheroplasts/analysis Succinate Dehydrogenase/analysis Ubiquinone/analysis
Chemicals
Bacteriochlorophylls Hemeproteins Ubiquinone Carotenoids Succinate Dehydrogenase Oxygen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Guillotin J
Reiss-Husson F
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32 references, click to expand
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Article Info
Journal
Archives of microbiology
Abbr.
Arch Microbiol
ISSN
0302-8933
DOI
10.1007/BF00447146
Published
1975-11-07
Pages
269-75
Language
English
Region
Germany
NLM ID
0410427
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00447146 View journal details
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