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PMID: 1082493 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Factors regulating macrophage production and growth: identity of colony-stimulating factor and macrophage growth factor.

The Journal of experimental medicine ·Vol. 143 ·No. 3 ·1976-03-01 ·Pages 631-47

Stanley ER, Cifone M, Heard PM, Defendi V

Abstract

The activities of a colony-stimulating factor (CSF), which stimulates granulocyte-macrophage colony formation by mouse hemopoietic cells, and macrophage growth factor (MGF), which stimulates proliferation of activated peritoneal macrophages, have been demonstrated by various criteria to reside in the same molecular species. These criteria include occurrence in various sources and copurification of the activities in mouse L-cell-conditioned medium as well as the biological, physicochemical, and antigenic properties of the activities of L-cell-conditioned medium. CSF and MGF activities of L-cell-conditioned medium are ascribable to a glycoprotein of mol wt approximately 60,000 which migrates electrophoretically with alpha-globulin. Human urinary CSF, which also possesses MGF activity, has similar properties and can be neutralized by antiserum to highly purified L-cell medium CSF. A procedure is described for the partial purification of material from L-cell medium that has activity at 1 ng/ml in both MGF and CSF assays.

MeSH Terms
Animals Antibody Formation Colony-Stimulating Factors/analysis,isolation & purification Culture Media Glycoproteins/analysis,isolation & purification Growth Substances/analysis,isolation & purification Hematopoietic Stem Cells/immunology Immune Sera Kinetics L Cells Macrophages/immunology Male Mice Molecular Weight
Chemicals
Colony-Stimulating Factors Culture Media Glycoproteins Growth Substances Immune Sera
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Stanley E R
Cifone M
Heard P M
Defendi V
References (44)
44 references, click to expand
  1. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.
    Ann N Y Acad Sci. 1964 Dec 28;121:404-27 PMID: 14240539
  2. Colony formation by mouse peritoneal exudate cells in vitro.
    Nat New Biol. 1973 Jun 6;243(127):176-7 PMID: 4514628
  3. Deoxyribonucleic acid synthesis in individual L-strain mouse cells. II. Effects of thymidine starvation.
    Biochim Biophys Acta. 1963 Jun 25;72:277-89 PMID: 13990346
  4. Studies of the culture of leukemic cells in vitro.
    Ann N Y Acad Sci. 1958 Dec 5;76(3):673-80 PMID: 13627893
  5. Polyacrylamide gel electrophoresis in a continuous molecular sieve gradient.
    Anal Biochem. 1968 Oct 24;25(1):347-62 PMID: 4178699
  6. Production of colony-stimulating factor in mixed leucocyte cultures.
    Immunology. 1974 May;26(5):1039-49 PMID: 4153023
  7. Neurotransmitter synthesis by neuroblastoma clones (neuroblast differentiation-cell culture-choline acetyltransferase-acetylcholinesterase-tyrosine hydroxylase-axons-dendrites).
    Proc Natl Acad Sci U S A. 1972 Jan;69(1):258-63 PMID: 4400294
  8. Properties of the mouse embryo conditioned medium factor(s) stimulationg colony formation by mouse bone marrow cells grown in vitro.
    J Cell Physiol. 1971 Oct;78(2):301-17 PMID: 4333458
  9. Infection and transformation of mouse peritoneal macrophages by simian virus 40.
    J Exp Med. 1971 Aug 1;134(2):335-50 PMID: 4326994
  10. Further studies on the factor in lung-conditioned medium stimulating granulocyte and monocyte colony formation in vitro.
    J Cell Physiol. 1974 Aug;84(1):147-58 PMID: 4210775
  11. Regulation of granulocyte and monocyte-macrophage proliferation by colony stimulating factor (CSF): a review.
    Exp Hematol. 1973;1(4):185-201 PMID: 4608467
  12. Letter: Growth of pulmonary alveolar macrophages in vitro.
    Nature. 1973 Sep 21;245(5421):150-2 PMID: 4582665
  13. Production of colony-stimulating factor in mitogen-stimulated lymphocyte cultures.
    J Immunol. 1974 Feb;112(2):502-10 PMID: 4591973
  14. Purification of mouse lung-conditioned medium colony stimulating factor (CSF).
    Proc Soc Exp Biol Med. 1974 May;146(1):218-21 PMID: 4597167
  15. Heterogeneity of molecules with low molecular weight isolated from media conditioned by human leukocytes and capable of stimulating colony formation by human granulopoietic progenitor cells.
    J Cell Physiol. 1974 Dec;84(3):383-96 PMID: 4530793
  16. Formation of eosinophilic-like granulocytic colonies by mouse bone marrow cells in vitro.
    J Cell Physiol. 1974 Oct;84(2):275-89 PMID: 4474181
  17. In vitro production of colony-stimulating activity. I. Exposure of mouse peritoneal cells to endotoxin.
    Cell Tissue Kinet. 1974 Jan;7(1):19-30 PMID: 4810547
  18. A low molecular weight factor in lung-conditioned medium stimulating granulocyte and monocyte colony formation in vitro.
    J Cell Physiol. 1973 Feb;81(1):11-23 PMID: 4685993
  19. Acute antigen-induced elevation of serum colony stimulating factor (CFS) levels.
    Immunology. 1971 Sep;21(3):427-36 PMID: 4936184
  20. Colony stimulating factor and the regulation of granulopoiesis and macrophage production.
    Fed Proc. 1975 Dec;34(13):2272-8 PMID: 1081456
  21. The isolation and properties of granulocytic colony-stimulating activities from medium conditioned by human peripheral leucocytes.
    Biochem J. 1975 May;148(2):209-17 PMID: 1156403
  22. Factors from mouse tissues stimulating colony growth of mouse bone marrow cells in vitro.
    Aust J Exp Biol Med Sci. 1971 Dec;49(6):595-603 PMID: 5170446
  23. Enzyme treatment of colony stimulating factor: evidence for a peptide component.
    Aust J Exp Biol Med Sci. 1971 Jun;49(3):281-90 PMID: 5119761
  24. Buffer combinations for mammalian cell culture.
    Science. 1971 Oct 29;174(4008):500-3 PMID: 5110427
  25. Regulation of DNA synthesis in mouse macrophages. II. Studies on mechanisms of action of the macrophage growth factor.
    Exp Cell Res. 1971 Apr;65(2):377-85 PMID: 5554036
  26. The molecular weight of colony-stimulating factor (CSF).
    Proc Soc Exp Biol Med. 1971 Jul;137(3):1029-31 PMID: 5560647
  27. Studies on colony formation in vitro by mouse bone marrow cells. II. Action of colony stimulating factor.
    J Cell Physiol. 1970 Aug;76(1):89-99 PMID: 5471044
  28. Group-specific adsorption of glycoproteins.
    Acta Chem Scand. 1970;24(5):1839-41 PMID: 5487824
  29. Studies on colony formation in vitro by mouse bone marrow cells. I. Continuous cluster formation and relation of clusters to colonies.
    J Cell Physiol. 1969 Dec;74(3):323-32 PMID: 5390860
  30. Stimulation of mouse bone marrow colony growth in vitro by conditioned medium.
    Aust J Exp Biol Med Sci. 1968 Oct;46(5):607-18 PMID: 5696493
  31. The cloning of normal "mast" cells in tissue culture.
    J Cell Physiol. 1965 Dec;66(3):319-24 PMID: 5884359
  32. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  33. A new human low molecular weight granulocyte colony stimulating activity.
    Blood. 1973 Sep;42(3):341-8 PMID: 4725599
  34. The growth of mouse bone marrow cells in vitro.
    Aust J Exp Biol Med Sci. 1966 Jun;44(3):287-99 PMID: 4164182
  35. A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis.
    Anal Biochem. 1967 Jul;20(1):150-4 PMID: 4166536
  36. Cyclic expression of a growth conditioning factor (MGF) on the cell surface.
    Nature. 1974 Nov 8;252(5479):151-3 PMID: 4420997
  37. Peritoneal exudate cells. I. Growth requirement of cells capable of forming colonies in soft agar.
    J Cell Physiol. 1974 Jun;83(3):369-78 PMID: 4857127
  38. Regulation of DNA synthesis in mouse macrophages. I. Sources, action and purification of the macrophage growing factor (MGF).
    Exp Cell Res. 1971 Mar;65(1):33-42 PMID: 4994337
  39. Characterization of the factor in L-cell conditioned medium capable of stimulating colony formation by mouse marrow cells in culture.
    J Cell Physiol. 1971 Apr;77(2):121-34 PMID: 5572424
  40. Dependence of macrophage growth in vitro upon interaction with other cell types.
    Wistar Inst Symp Monogr. 1967;7:67-85 PMID: 5586010
  41. Partial purification and some properties of the factor in normal and leukaemic human urine stimulating mouse bone marrow colony growth in vitro.
    Aust J Exp Biol Med Sci. 1969 Aug;47(4):467-83 PMID: 4309905
  42. Two types of ribosome in mouse-hamster hybrid cells.
    Nat New Biol. 1971 Mar 10;230(10):52-4 PMID: 5279808
  43. Release of bone marrow colony stimulating activity during immunological reactions in vitro.
    Nat New Biol. 1973 Aug 8;244(136):175-6 PMID: 4579671
  44. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.
    Biochem J. 1963 Oct;89:114-23 PMID: 14097352
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.143.3.631
Published
1976-03-01
Pages
631-47
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2190132
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.143.3.631 PMC Full Text View journal details
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