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PMID: 1083200 Published · ppublish English Journal Article

Susceptibility of Haemophilus influenza to ampicillin as determined by use of a modified, one-minute beta-lactamase test.

Antimicrobial agents and chemotherapy ·Vol. 9 ·No. 1 ·1976-01-00 ·Pages 196-8

Escamilla J

Abstract

Fifty-seven cultures of Haemophilus influenzae were tested for production of beta-lactamase by use of a modified, 1-min test. Only the 24 cultures that were ampicillin resistant, whether type b or not, yielded positive results for the enzyme; all ampicillin-susceptible isolates were uniformly negative. The beta-lactamase procedure is simple to perform and requires only commonly available reagents.

MeSH Terms
Ampicillin/pharmacology Haemophilus influenzae/drug effects,enzymology Microbial Sensitivity Tests/methods Penicillinase Time Factors
Chemicals
Ampicillin Penicillinase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Escamilla J
References (8)
8 references, click to expand
  1. Ampicillin resistance in Haemophilus influenzae as determined by a rapid test for beta-lactamase production.
    Antimicrob Agents Chemother. 1974 Nov;6(5):653-4 PMID: 15825323
  2. Beta-lactamase activity in ampicillin-resistant Haemophilus influenzae.
    Antimicrob Agents Chemother. 1974 Nov;6(5):625-9 PMID: 15825317
  3. Antimicrobial susceptibility of Haemophilus influenzae.
    Antimicrob Agents Chemother. 1974 Nov;6(5):620-4 PMID: 15825316
  4. Clinical, bacteriological, and immunological characterisation of ampicillin-resistant Haemophilus influenzae type B.
    Lancet. 1974 Aug 3;2(7875):257-9 PMID: 4136145
  5. Haemophilus influenzae type B resistant to ampicillin. A report of two cases.
    JAMA. 1974 Jul 15;229(3):298-301 PMID: 4546094
  6. Rapid capillary tube method for detecting penicillin resistance in Staphylococcus aureus.
    Appl Microbiol. 1972 Mar;23(3):649-50 PMID: 4482067
  7. Antimicrobial susceptibility of Hemophilus influenzae.
    Pediatrics. 1970 May;45(5):827-38 PMID: 5309847
  8. Iodometric detection of Haemophilus influenzae beta-lactamase: rapid presumptive test for ampicillin resistance.
    Antimicrob Agents Chemother. 1975 Mar;7(3):265-70 PMID: 1079712
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.9.1.196
Published
1976-01-00
Pages
196-8
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC429498
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.9.1.196 PMC Full Text View journal details
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