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PMID: 10833030 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Composition of N-linked carbohydrates from ovalbumin and co-purified glycoproteins.

Journal of the American Society for Mass Spectrometry ·Vol. 11 ·No. 6 ·2000-06-00 ·Pages 564-71

Harvey DJ, Wing DR, Küster B, Wilson IB

Abstract

Analysis of commercial samples of chicken ovalbumin by reversed-phase high performance liquid chromatography and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) showed the presence of several other co-purifying glycoproteins. These were isolated, subjected to tryptic digestion, and two of them were identified as ovomucoid and chicken riboflavin binding-protein following database matching of the peptide masses obtained by MALDI. The N-linked glycans were released from the glycoproteins and their structures were examined by MALDI-MS in combination with exoglycosidase digestion. Ovalbumin was found to be glycosylated mainly with high-mannose and hybrid structures, consistent with profiles obtained on the intact glycoprotein by electrospray. The other glycoproteins contained mainly larger, complex glycans with up to five antennae, many of which had earlier been associated with ovalbumin.

MeSH Terms
Carbohydrates/chemistry,isolation & purification Chromatography, High Pressure Liquid Electrophoresis, Polyacrylamide Gel Glycoproteins/chemistry,isolation & purification Glycoside Hydrolases/chemistry Hydrazines/chemistry Hydrolysis Mass Spectrometry Ovalbumin/chemistry,isolation & purification Peptides/chemistry Polysaccharides/chemistry,isolation & purification Trypsin
Chemicals
Carbohydrates Glycoproteins Hydrazines Peptides Polysaccharides Ovalbumin Glycoside Hydrolases Trypsin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Harvey D J
Department of Biochemistry, Oxford Glycobiology Institute, United Kingdom. [email protected]
Wing D R
Küster B
Wilson I B
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23 references, click to expand
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Article Info
Journal
Journal of the American Society for Mass Spectrometry
Abbr.
J Am Soc Mass Spectrom
ISSN
1044-0305
DOI
10.1016/S1044-0305(00)00122-7
Email
[email protected]
Published
2000-06-00
Pages
564-71
Language
English
Region
United States
NLM ID
9010412
Subset
IM
External Links
PubMed source DOI: 10.1016/S1044-0305(00)00122-7 View journal details
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