Home LiteratureArticle Details
PMID: 1083895 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Density and distribution of tetrodotoxin receptors in normal and detubulated frog sartorius muscle.

The Journal of general physiology ·Vol. 67 ·No. 4 ·1976-04-00 ·Pages 399-416

Jaimovich E, Venosa RA, Shrager P, Horowicz P

Abstract

Tetrodotoxin (TTX) binding was measured in muscles which were either in normal condition or which had been "detubulated" by glycerol-induced osmotic shock. In both cases the binding of TTX was found to saturate at high TTX concentrations. Maximum binding in normal fibers was 35 pmol/g wet weight, and that figure was reduced to 16 pmol/g after glycerol treatment. The dissociation constant for binding to the surface membrane was 3 nM, which is the range of values obtained by electrophysiological measurements of the effect of TTX on the maximum rate of rise of the action potential. The results suggest that the dissociation constant in the transverse tubules may be higher than that in the surface. Control experiments indicate that the effects of glycerol treatment are limited to the accessibility of the receptors to the toxin and result in no alteration of the affinity of the binding site. TTX receptors in the transverse tubules may be recovered after glycerol treatment by homogenization of the fibers. The measurements suggest that the density of sodium channels in surface membrane is about 175/muM2 and that in the transverse tubular membrane is 41-52/mum2.

MeSH Terms
Action Potentials/drug effects Animals Astacoidea Biological Assay Dose-Response Relationship, Drug Glycerol/pharmacology Models, Biological Muscles/metabolism,physiology Rana pipiens Receptors, Drug/drug effects Tetrodotoxin/metabolism,pharmacology
Chemicals
Receptors, Drug Tetrodotoxin Glycerol
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jaimovich E
Venosa R A
Shrager P
Horowicz P
References (34)
34 references, click to expand
  1. Frog skeletal muscle fibers: changes in electrical properties after disruption of transverse tubular system.
    Science. 1967 Dec 29;158(3809):1700-1 PMID: 6070028
  2. Selective disruption of the sarcotubular system in frog sartorius muscle. A quantitative study with exogenous peroxidase as a marker.
    J Cell Biol. 1968 Nov;39(2):451-67 PMID: 5692585
  3. Capacitance of the surface and transverse tubular membrane of frog sartorius muscle fibers.
    J Gen Physiol. 1969 Mar;53(3):265-78 PMID: 5767332
  4. Tetrodotoxin binding to normal depolarized frog muscle and the conductance of a single sodium channel.
    J Physiol. 1975 May;247(2):483-509 PMID: 1080198
  5. The binding of tritiated tetrodotoxin to squid giant axons.
    Philos Trans R Soc Lond B Biol Sci. 1975 Jun 10;270(908):349-52 PMID: 238233
  6. The purity of tritiated tetrodotoxin as determined by bioassay.
    Philos Trans R Soc Lond B Biol Sci. 1975 Jun 10;270(908):337-48 PMID: 238232
  7. Partial characterization of a tetrodotoxin-binding component from nerve membrane.
    Proc Natl Acad Sci U S A. 1972 Dec;69(12):3634-7 PMID: 4509325
  8. Action potential in the transverse tubules and its role in the activation of skeletal muscle.
    J Gen Physiol. 1974 Feb;63(2):257-78 PMID: 4812638
  9. Inhibition of the receptor for tetrodotoxin in nerve membranes by reagents modifying carboxyl groups.
    Biochim Biophys Acta. 1973 Aug 9;318(1):141-6 PMID: 4747072
  10. A convenient method for repeated intracellular recording of action potentials from the same muscle fibre without membrane damage.
    Pflugers Arch. 1972;334(3):276-8 PMID: 4676258
  11. The kinetics of the interaction between tetrodotoxin and mammalian nonmyelinated nerve fibers.
    Mol Pharmacol. 1972 May;8(3):285-92 PMID: 5040931
  12. Mechanism of action of tetrodotoxin and saxitoxin on excitable membranes.
    Fed Proc. 1972 May-Jun;31(3):1124-32 PMID: 5032476
  13. Equilibrium and kinetic properties of the interaction between tetrodotoxin and the excitable membrane of the squid giant axon.
    J Gen Physiol. 1970 Mar;55(3):309-35 PMID: 5520505
  14. The role of sodium current in the radial spread of contraction in frog muscle fibers.
    J Gen Physiol. 1970 Jun;55(6):703-15 PMID: 5424374
  15. Pharmacological modifications of the sodium channels of frog nerve.
    J Gen Physiol. 1968 Feb;51(2):199-219 PMID: 5641635
  16. The sarcoplasmic reticulum and transverse tubules of the frog's sartorius.
    J Cell Biol. 1965 Jun;25(3):Suppl:209-31 PMID: 5840799
  17. Studies on plant amylases: The effect of starch concentration upon the velocity of hydrolysis by the amylase of germinated barley.
    Biochem J. 1932;26(5):1406-21 PMID: 16744959
  18. The binding of labelled tetrodotoxin to non-myelinated nerve fibres.
    J Physiol. 1972 Dec;227(1):95-126 PMID: 4646588
  19. Analysis of the membrane capacity in frog muscle.
    J Physiol. 1972 Feb;221(1):121-36 PMID: 5016975
  20. Sodium dependence of the inward spread of activation in isolated twitch muscle fibres of the frog.
    J Physiol. 1972 Jun;223(2):507-23 PMID: 4537711
  21. Kinetic properties of the sodium current in striated muscle fibres on the basis of the Hodgkin-Huxley theory.
    J Physiol. 1972 Dec;227(2):419-31 PMID: 4647253
  22. Reconstruction of the action potential of frog sartorius muscle.
    J Physiol. 1973 Nov;235(1):103-31 PMID: 4778131
  23. The rate of action of tetrodotoxin on myelinated nerve fibres of Xenopus laevis and Rana esculenta.
    J Physiol. 1973 Aug;233(1):167-94 PMID: 4543328
  24. Ionic diffusion delays in the transverse tubules of frog twitch muscle fibres.
    J Physiol. 1973 Mar;229(2):547-57 PMID: 4724837
  25. Morphology and accessibility of the 'transverse' tubular system in frog sartorius muscle after glycerol treatment.
    J Membr Biol. 1973;14(3):197-212 PMID: 4130465
  26. The maintenance of resting potentials in glycerol-treated muscle fibres.
    J Physiol. 1971 May;215(1):95-102 PMID: 5579685
  27. Voltage clamp experiments in striated muscle fibres.
    J Physiol. 1970 Jul;208(3):607-44 PMID: 5499787
  28. Inward spread of activation in vertebrate muscle fibres.
    J Physiol. 1971 Feb;212(3):777-99 PMID: 5557071
  29. The binding of tetrodotoxin to nerve membranes.
    J Physiol. 1971 Feb;213(1):235-54 PMID: 5575342
  30. Kinetics and steady-state properties of the charged system controlling sodium conductance in the squid giant axon.
    J Physiol. 1974 Jun;239(2):393-434 PMID: 4414038
  31. The binding of tetrodotoxin and alpha-bungarotoxin to normal and denervated mammalian muscle.
    J Physiol. 1974 Jul;240(1):199-226 PMID: 4854666
  32. Inward movement of sodium ions in resting and stimulated frog's sartorius muscle.
    J Physiol. 1974 Aug;241(1):155-73 PMID: 4547580
  33. An upper limit to the number of sodium channels in nerve membrane?
    J Physiol. 1967 Jan;188(1):99-105 PMID: 6032201
  34. Effects on sodium efflux of treating frog sartorius muscles with hypertonic glycerol solutions.
    J Membr Biol. 1973 Dec 6;14(1):33-56 PMID: 4544049
Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.67.4.399
Published
1976-04-00
Pages
399-416
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2214919
Subset
IM
External Links
PubMed source DOI: 10.1085/jgp.67.4.399 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2