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PMID: 10848580 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

PKR stimulates NF-kappaB irrespective of its kinase function by interacting with the IkappaB kinase complex.

Molecular and cellular biology ·Vol. 20 ·No. 13 ·2000-07-00 ·Pages 4532-42

Bonnet MC, Weil R, Dam E, Hovanessian AG, Meurs EF

Abstract

The interferon (IFN)-induced double-stranded RNA-activated protein kinase PKR mediates inhibition of protein synthesis through phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (eIF2alpha) and is also involved in the induction of the IFN gene through the activation of the transcription factor NF-kappaB. NF-kappaB is retained in the cytoplasm through binding to its inhibitor IkappaBalpha. The critical step in NF-kappaB activation is the phosphorylation of IkappaBalpha by the IkappaB kinase (IKK) complex. This activity releases NF-kappaB from IkappaBalpha and allows its translocation to the nucleus. Here, we have studied the ability of PKR to activate NF-kappaB in a reporter assay and have shown for the first time that two catalytically inactive PKR mutants, PKR/KR296 and a deletion mutant (PKR/Del42) which lacks the potential eIF2alpha-binding domain, can also activate NF-kappaB. This result indicated that NF-kappaB activation by PKR does not require its kinase activity and that it is independent of the PKR-eIF2alpha relationship. Transfection of either wild-type PKR or catalytically inactive PKR in PKR(0/0) mouse embryo fibroblasts resulted in the activation of the IKK complex. By using a glutathione S-transferase pull-down assay, we showed that PKR interacts with the IKKbeta subunit of the IKK complex. This interaction apparently does not require the integrity of the IKK complex, as it was found to occur with extracts from cells deficient in the NF-kappaB essential modulator, one of the components of the IKK complex. Therefore, our results reveal a novel pathway by which PKR can modulate the NF-kappaB signaling pathway without using its kinase activity.

MeSH Terms
Amino Acid Sequence Animals Enzyme Activation Fibroblasts Gene Expression Regulation, Enzymologic Glutathione Transferase/genetics,metabolism Humans I-kappa B Kinase Interferons/metabolism Mice Mice, Mutant Strains Molecular Sequence Data Mutation NF-kappa B/genetics,metabolism Protein Serine-Threonine Kinases/metabolism Recombinant Proteins/genetics,metabolism Response Elements Signal Transduction eIF-2 Kinase/genetics,metabolism
Chemicals
NF-kappa B Recombinant Proteins Interferons Glutathione Transferase Protein Serine-Threonine Kinases eIF-2 Kinase CHUK protein, human Chuk protein, mouse I-kappa B Kinase IKBKB protein, human IKBKE protein, human Ikbkb protein, mouse Ikbke protein, mouse
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bonnet M C
Unité de Virologie et d'Immunologie Cellulaire, URA CNRS 1930, Institut Pasteur, 75724 Paris Cedex 15, France.
Weil R
Dam E
Hovanessian A G
Meurs E F
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.20.13.4532-4542.2000
Published
2000-07-00
Pages
4532-42
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC85837
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.20.13.4532-4542.2000 PMC Full Text View journal details
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