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PMID: 10859180 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The role of pyruvate dehydrogenase and acetyl-coenzyme A synthetase in fatty acid synthesis in developing Arabidopsis seeds.

Plant physiology ·Vol. 123 ·No. 2 ·2000-06-00 ·Pages 497-508

Ke J, Behal RH, Back SL, Nikolau BJ, Wurtele ES, Oliver DJ

Abstract

Acetyl-coenzyme A (acetyl-CoA) formed within the plastid is the precursor for the biosynthesis of fatty acids and, through them, a range of important biomolecules. The source of acetyl-CoA in the plastid is not known, but two enzymes are thought to be involved: acetyl-CoA synthetase and plastidic pyruvate dehydrogenase. To determine the importance of these two enzymes in synthesizing acetyl-CoA during lipid accumulation in developing Arabidopsis seeds, we isolated cDNA clones for acetyl-CoA synthetase and for the ptE1alpha- and ptE1beta-subunits of plastidic pyruvate dehydrogenase. To our knowledge, this is the first reported acetyl-CoA synthetase sequence from a plant source. The Arabidopsis acetyl-CoA synthetase preprotein has a calculated mass of 76,678 D, an apparent plastid targeting sequence, and the mature protein is a monomer of 70 to 72 kD. During silique development, the spatial and temporal patterns of the ptE1beta mRNA level are very similar to those of the mRNAs for the plastidic heteromeric acetyl-CoA carboxylase subunits. The pattern of ptE1beta mRNA accumulation strongly correlates with the formation of lipid within the developing embryo. In contrast, the level of mRNA for acetyl-CoA synthetase does not correlate in time and space with lipid accumulation. The highest level of accumulation of the mRNA for acetyl-CoA synthetase during silique development is within the funiculus. These mRNA data suggest a predominant role for plastidic pyruvate dehydrogenase in acetyl-CoA formation during lipid synthesis in seeds.

MeSH Terms
Acetate-CoA Ligase/chemistry,genetics,metabolism Amino Acid Sequence Arabidopsis/embryology,enzymology Cloning, Molecular Fatty Acids/biosynthesis In Situ Hybridization Molecular Sequence Data Plastids/enzymology Pyruvate Dehydrogenase Complex/metabolism RNA, Messenger/genetics,metabolism Seeds/enzymology,growth & development Sequence Homology, Amino Acid
Chemicals
Fatty Acids Pyruvate Dehydrogenase Complex RNA, Messenger Acetate-CoA Ligase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ke J
Department of Botany, Biophysics, and Molecular Biology, Iowa State University, Ames 50011, USA.
Behal R H
Back S L
Nikolau B J
Wurtele E S
Oliver D J
References (49)
49 references, click to expand
  1. A workbench for multiple alignment construction and analysis.
    Proteins. 1991;9(3):180-90 PMID: 2006136
  2. Pyruvate dehydrogenase complex from higher plant mitochondria and proplastids.
    Plant Physiol. 1977 May;59(5):842-8 PMID: 16659953
  3. Purification and Characterization of the Pea Chloroplast Pyruvate Dehydrogenase Complex : A Source of Acetyl-CoA and NADH for Fatty Acid Biosynthesis.
    Plant Physiol. 1985 Mar;77(3):571-7 PMID: 16664100
  4. Coordinate regulation of the nuclear and plastidic genes coding for the subunits of the heteromeric acetyl-coenzyme A carboxylase.
    Plant Physiol. 2000 Apr;122(4):1057-71 PMID: 10759501
  5. Correlation of ATP/citrate lyase activity with lipid accumulation in developing seeds of Brassica napus L.
    Lipids. 1997 Jan;32(1):7-12 PMID: 9075187
  6. Lipid biosynthesis.
    Plant Cell. 1995 Jul;7(7):957-70 PMID: 7640528
  7. Citrate cleavage enzymes from developing soybean cotyledons: incorporation of citrate carbon into Fatty acids.
    Plant Physiol. 1975 Jan;55(1):69-72 PMID: 16659030
  8. Acetate is the preferred substrate for long-chain fatty acid synthesis in isolated spinach chloroplasts.
    Biochem J. 1979 Dec 15;184(3):565-9 PMID: 540048
  9. Malate- and pyruvate-dependent Fatty Acid synthesis in leucoplasts from developing castor endosperm.
    Plant Physiol. 1992 Apr;98(4):1233-8 PMID: 16668781
  10. Subcellular localization of acetyl-CoA synthetase in leaf protoplasts of Spinacia oleracea.
    Arch Biochem Biophys. 1981 Jul;209(2):441-50 PMID: 6117251
  11. Genomic sequencing.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 PMID: 6326095
  12. Partial purification of rat liver cytoplasmic acetyl-CoA synthetase; characterization of some properties.
    Int J Biochem. 1984;16(8):875-81 PMID: 6147283
  13. Purification and properties of acetyl coenzyme A synthetase from bakers' yeast.
    J Biol Chem. 1977 Jan 25;252(2):504-7 PMID: 13070
  14. Cloning of the cDNA and genomic clones for glutathione synthetase from Arabidopsis thaliana and complementation of a gsh2 mutant in fission yeast.
    Plant Mol Biol. 1996 Sep;31(6):1093-104 PMID: 8914526
  15. Plastidic Isoprenoid Synthesis during Chloroplast Development : Change from Metabolic Autonomy to a Division-of-Labor Stage.
    Plant Physiol. 1990 Jul;93(3):1121-7 PMID: 16667567
  16. Sequence analysis of firefly luciferase family reveals a conservative sequence motif.
    Protein Seq Data Anal. 1991 Aug;4(2):111-7 PMID: 1946328
  17. Domain structure of mitochondrial and chloroplast targeting peptides.
    Eur J Biochem. 1989 Apr 1;180(3):535-45 PMID: 2653818
  18. beta-Carotene Synthesis in Isolated Spinach Chloroplasts : Its Tight Linkage to Photosynthetic Carbon Metabolism.
    Plant Physiol. 1987 Aug;84(4):1233-7 PMID: 16665589
  19. Pyruvate Dehydrogenase Complex from Chloroplasts of Pisum sativum L.
    Plant Physiol. 1979 Dec;64(6):1099-103 PMID: 16661100
  20. Origin of acetate in spinach leaf cell.
    Plant Physiol. 1982 Apr;69(4):897-903 PMID: 16662316
  21. The rf2 nuclear restorer gene of male-sterile T-cytoplasm maize.
    Science. 1996 May 31;272(5266):1334-6 PMID: 8650543
  22. Sulfate transport and assimilation in plants
    Plant Physiol. 1999 Jul;120(3):637-44 PMID: 10398698
  23. Lipid biosynthesis from [14C]bicarbonate, [2(-14)C]pyruvate and [1(-14)C]acetate during photosynthesis by isolated spinach chloroplasts.
    FEBS Lett. 1977 May 15;77(2):164-8 PMID: 862916
  24. Isolation and characterization of acetyl-coenzyme A synthetase from Methanothrix soehngenii.
    J Bacteriol. 1989 Oct;171(10):5430-5 PMID: 2571608
  25. Purification and properties of acetyl-CoA synthetase from Bradyrhizobium japonicum bacteroids.
    Biochem J. 1990 Apr 1;267(1):179-83 PMID: 1970239
  26. Intracellular location of ATP citrate lyase in leaves of Pisum sativum L.
    Planta. 1985 Feb;163(2):290-4 PMID: 24249352
  27. Comparison of acetate- and pyruvate-dependent fatty-acid synthesis by spinach chloroplasts.
    Planta. 1989 Mar;177(3):417-21 PMID: 24212436
  28. The biosynthesis of erucic acid in developing embryos of brassica rapa
    Plant Physiol. 1998 Sep;118(1):183-90 PMID: 9733537
  29. The capacity of plastids from developing pea cotyledons to synthesise acetyl CoA.
    Planta. 1988 Feb;173(2):172-82 PMID: 24226397
  30. Chloroplast transit peptides. The perfect random coil?
    FEBS Lett. 1991 Jan 14;278(1):1-3 PMID: 1993469
  31. cRACE: a simple method for identification of the 5' end of mRNAs.
    Nucleic Acids Res. 1995 Sep 25;23(18):3796-7 PMID: 7479016
  32. Structure of the CAC1 gene and in situ characterization of its expression. The Arabidopsis thaliana gene coding for the biotin-containing subunit of the plastidic acetyl-coenzyme A carboxylase.
    Plant Physiol. 1997 Feb;113(2):357-65 PMID: 9046589
  33. Is Acetylcarnitine a Substrate for Fatty Acid Synthesis in Plants?
    Plant Physiol. 1993 Apr;101(4):1157-1162 PMID: 12231767
  34. The molecular weight and thiol residues of acetyl-coenzyme A synthetase from ox heart mitochondria.
    Biochem J. 1973 May;133(1):23-36 PMID: 4737256
  35. On the control of long-chain-fatty acid synthesis in isolated intact spinach (Spinacia oleracea) chloroplasts.
    Biochem J. 1979 Nov 15;184(2):193-202 PMID: 534525
  36. Fatty-acid synthesis in chloroplasts from mustard (Sinapis alba L.) cotyledons: formation of acetyl coenzyme A by intraplastid glycolytic enzymes and a pyruvate dehydrogenase complex.
    Planta. 1986 Dec;169(4):481-9 PMID: 24232754
  37. Studies of the acetyl coenzyme A synthetase reaction. 3. Evidence of a double requirement for divalent cations.
    J Biol Chem. 1965 Nov;240(11):4164-9 PMID: 5845819
  38. High rates of [1-14C]acetate incorporation into the lipid of isolated spinach chloroplasts.
    Biochem J. 1976 Sep 15;158(3):593-601 PMID: 985452
  39. Identification and Characterization of Mitochondrial Acetyl-Coenzyme A Hydrolase from Pisum sativum L. Seedlings.
    Plant Physiol. 1990 Sep;94(1):20-7 PMID: 16667687
  40. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
    Nucleic Acids Res. 1994 Nov 11;22(22):4673-80 PMID: 7984417
  41. Molecular cloning of the biotinylated subunit of 3-methylcrotonyl-coenzyme A carboxylase of Arabidopsis thaliana.
    Plant Physiol. 1995 Mar;107(3):1013-4 PMID: 7716229
  42. Aldehyde dehydrogenase in tobacco pollen.
    Plant Mol Biol. 1997 Oct;35(3):355-65 PMID: 9349259
  43. Molecular cloning and characterization of the cDNA coding for the biotin-containing subunit of the chloroplastic acetyl-coenzyme A carboxylase.
    Plant Physiol. 1995 Oct;109(2):619-25 PMID: 7480350
  44. On the assay of acetyl-CoA synthetase activity in chloroplasts and leaf extracts.
    Anal Biochem. 1994 Jan;216(1):77-82 PMID: 7907845
  45. Biochemical and molecular characterization of fumarase from plants: purification and characterization of the enzyme--cloning, sequencing, and expression of the gene.
    Arch Biochem Biophys. 1997 Dec 1;348(1):65-74 PMID: 9390175
  46. Cloning and molecular analyses of the Arabidopsis thaliana plastid pyruvate dehydrogenase subunits.
    Biochim Biophys Acta. 1997 Oct 20;1321(3):200-6 PMID: 9393637
  47. Aerobic fermentation during tobacco pollen development.
    Plant Mol Biol. 1997 Oct;35(3):343-54 PMID: 9349258
  48. Preparation of physiologically active chloroplasts from Arabidopsis.
    Methods Mol Biol. 1998;82:43-8 PMID: 9664410
  49. ATP Citrate Lyase from Germinating Castor Bean Endosperm: Localization and some Properties.
    Plant Physiol. 1979 Apr;63(4):687-91 PMID: 16660792
Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.123.2.497
Published
2000-06-00
Pages
497-508
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC59018
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.123.2.497 PMC Full Text View journal details
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