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PMID: 108667 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Antibody to B. subtilis DNA polymerase III: use in enzyme purification and examination of homology among replication-specific DNA polymerases.

Nucleic acids research ·Vol. 6 ·No. 3 ·1979-03-00 ·Pages 1203-19

Barnes MH, Brown NC

Abstract

Bacillus subtilis DNA polymerase III (pol III), an arylhydrazinopyrimidine-sensitive, replication-specific enzyme, was used to generate a non-precipitating rabbit antibody which specifically inhibited pol III activity in vitro. The antibody was used to examine structural relationships among several DNA polymerases, and it was linked covalently to agarose; the antibody:agarose was employed to develop a rapid, selective method of purification of catalytically active B. subtilis pol III.

MeSH Terms
Animals Antibodies Antigen-Antibody Reactions Bacillus subtilis/enzymology DNA Polymerase III/immunology,isolation & purification DNA Replication DNA-Directed DNA Polymerase/isolation & purification Kinetics Molecular Weight Rabbits/immunology Species Specificity
Chemicals
Antibodies DNA Polymerase III DNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Barnes M H
Brown N C
References (20)
20 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.3.1203
Published
1979-03-00
Pages
1203-19
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327764
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/6.3.1203 PMC Full Text View journal details
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