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PMID: 10869433 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A regulatory shortcut between the Snf1 protein kinase and RNA polymerase II holoenzyme.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 97 ·No. 14 ·2000-07-05 ·Pages 7916-20

Kuchin S, Treich I, Carlson M

Abstract

RNA polymerase II holoenzymes respond to activators and repressors that are regulated by signaling pathways. Here we present evidence for a "shortcut" mechanism in which the Snf1 protein kinase of the glucose signaling pathway directly regulates transcription by the yeast holoenzyme. In response to glucose limitation, the Snf1 kinase stimulates transcription by holoenzyme that has been artificially recruited to a reporter by a LexA fusion to a holoenzyme component. We show that Snf1 interacts physically with the Srb/mediator proteins of the holoenzyme in both two-hybrid and coimmunoprecipitation assays. We also show that a catalytically hyperactive Snf1, when bound to a promoter as a LexA fusion protein, activates transcription in a glucose-regulated manner; moreover, this activation depends on the integrity of the Srb/mediator complex. These results suggest that direct regulatory interactions between signal transduction pathways and RNA polymerase II holoenzyme provide a mechanism for transcriptional control in response to important signals.

MeSH Terms
Bacterial Proteins/metabolism Cyclin-Dependent Kinase 8 Cyclin-Dependent Kinases/metabolism Cyclins/metabolism Fungal Proteins/metabolism Gene Expression Regulation, Fungal Glucose/pharmacology Holoenzymes/metabolism Mediator Complex Models, Genetic Precipitin Tests Promoter Regions, Genetic Protein Binding Protein Serine-Threonine Kinases/metabolism RNA Polymerase II/metabolism Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Serine Endopeptidases/metabolism Signal Transduction Trans-Activators Transcription Factors/metabolism Transcriptional Activation Two-Hybrid System Techniques
Chemicals
Bacterial Proteins Cyclins Fungal Proteins Holoenzymes LexA protein, Bacteria Mediator Complex SIN4 protein, S cerevisiae Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors SNF1-related protein kinases SSN8 protein, S cerevisiae Protein Serine-Threonine Kinases Cyclin-Dependent Kinase 8 Cyclin-Dependent Kinases SSN3 protein, S cerevisiae RNA Polymerase II Serine Endopeptidases Glucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kuchin S
Department of Genetics and Development and Department of Microbiology, Columbia University, New York, NY 10032, USA.
Treich I
Carlson M
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38 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.140109897
Published
2000-07-05
Pages
7916-20
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC16645
Subset
IM
Grants
NIGMS NIH HHS · R01 GM047259 · United States
NCI NIH HHS · R01 CA52559 · United States
NCI NIH HHS · T32CA 09109 · United States
External Links
PubMed source DOI: 10.1073/pnas.140109897 PMC Full Text View journal details
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