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PMID: 10880478 Published · ppublish English Journal Article

Abnormal turning behavior in Drosophila larvae. Identification and molecular analysis of scribbler (sbb).

Genetics ·Vol. 155 ·No. 3 ·2000-07-00 ·Pages 1161-74

Yang P, Shaver SA, Hilliker AJ, Sokolowski MB

Abstract

Our genetic dissection of behavior has isolated scribbler (sbb), a vital gene that encodes a novel protein expressed in the embryonic and larval nervous systems and in the imaginal discs. Larvae with mutations in sbb exhibit abnormally high amounts of turning behavior in the absence of food. sbb is a large gene spanning >50 kb of genomic DNA with four major developmentally regulated transcripts. Transgenic rescue of scribbler behavior was demonstrated by targeting expression of a normal sbb transgene (sbb(+)) expressing one of the major transcripts to the nervous system. The vital function of sbb was restored by ubiquitous expression of this transgene throughout development.

MeSH Terms
Amino Acid Sequence/genetics Animals Base Sequence/genetics Behavior, Animal Central Nervous System/metabolism Drosophila Proteins Drosophila melanogaster/genetics Embryo, Nonmammalian/metabolism Female Gene Expression Regulation, Developmental Gene Targeting Genes, Lethal/genetics Insect Proteins/genetics,metabolism Larva/metabolism Locomotion/genetics Male Molecular Sequence Data Nerve Growth Factors Neurons/metabolism Organisms, Genetically Modified Phenotype Physical Chromosome Mapping RNA, Messenger/biosynthesis Sequence Analysis, DNA Transgenes Zinc Fingers/genetics
Chemicals
Drosophila Proteins Insect Proteins Nerve Growth Factors RNA, Messenger sbb protein, Drosophila
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yang P
Department of Zoology, University of Toronto, Mississauga, Ontario L5L 1C6, Canada.
Shaver S A
Hilliker A J
Sokolowski M B
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/155.3.1161
Published
2000-07-00
Pages
1161-74
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1461145
Subset
IM
Databases
GENBANK
AF247562, AF247563
External Links
PubMed source DOI: 10.1093/genetics/155.3.1161 PMC Full Text View journal details
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