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PMID: 10889235 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cell-specific and conditional expression of caffeoyl-coenzyme A-3-O-methyltransferase in poplar.

Plant physiology ·Vol. 123 ·No. 3 ·2000-07-00 ·Pages 853-67

Chen C, Meyermans H, Burggraeve B, De Rycke RM, Inoue K, De Vleesschauwer V, Steenackers M, Van Montagu MC, Engler GJ, Boerjan WA

Abstract

Caffeoyl coenzyme A-3-O-methyltransferase (CCoAOMT) plays an important role in lignin biosynthesis and is encoded by two genes in poplar (Populus trichocarpa). Here, we describe the expression pattern conferred by the two CCoAOMT promoters when fused to the gus-coding sequence in transgenic poplar (Populus tremula x Populus alba). Both genes were expressed similarly in xylem and differentially in phloem. In xylem, expression was preferentially observed in vessels and contact rays, whereas expression was barely detectable in storage rays and fibers, suggesting different routes to monolignol biosynthesis in the different xylem types. Furthermore, after wounding, fungal infection, and bending, the expression of both genes was induced concomitantly with de novo lignin deposition. Importantly, upon bending and leaning of the stem, the cell-specific expression pattern was lost, and both genes were expressed in all cell types of the xylem. CCoAOMT promoter activity correlated well with the presence of the CCoAOMT protein, as shown by immunolocalization. These expression data may explain, at least in part, the heterogeneity in lignin composition that is observed between cell types and upon different environmental conditions.

MeSH Terms
Blotting, Western Fungi Gene Expression Regulation, Plant Lignin/biosynthesis Methyltransferases/genetics Microscopy, Electron Plant Stems/cytology,metabolism,ultrastructure Plants, Genetically Modified Promoter Regions, Genetic Rosales/cytology,enzymology,genetics,microbiology
Chemicals
Lignin Methyltransferases caffeoyl-CoA O-methyltransferase
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Chen C
Vakgroep Moleculaire Genetica & Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Gent, Belgium.
Meyermans H
Burggraeve B
De Rycke R M
Inoue K
De Vleesschauwer V
Steenackers M
Van Montagu M C
Engler G J
Boerjan W A
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.123.3.853
Published
2000-07-00
Pages
853-67
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC59049
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.123.3.853 PMC Full Text View journal details
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