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PMID: 1089598 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Isolation of mitogenic and adjuvant active fractions from various species of Nocardiae.

Infection and immunity ·Vol. 11 ·No. 2 ·1975-02-00 ·Pages 257-64

Ciorbaru R, Adam A, Petit JF, Lederer E, Bona C, Chedid L

Abstract

Delipidated lysozyme digests of Nocardia opaca, N. corallina, and N. rubra have been fractionated by Sephadex filtration. The mitogenic and adjuvant activities of the fractions thus obtained have been investigated. All fractions are mitogenic except the last fraction of N.rubra, but the N. opaca products induce a stronger stimulation of mouse spleen lymphocytes than the corresponding fractions of the two other species. The activity of the first Sephadex fractions of each strain has been compared to other mitogens (concanavalin A, lipopolysaccharide). All fractions are adjuvant, although one of them, the last Sephadex fraction of N. rubra, does not contain peptidoglycan; its activity must thus be attributed to another kind of molecule. Fractionation of the first Sephadex fraction of N. opaca by centrifugation in glacial acetic acid led to a separation of adjuvant and mitogenic activities.

MeSH Terms
Adjuvants, Immunologic/isolation & purification Amino Acids/analysis Amino Sugars/analysis Animals Bacteriological Techniques Chromatography, Gel Female Guinea Pigs Lipids/isolation & purification Lymphocyte Activation Lymphocytes/immunology Mice Mice, Inbred AKR Mice, Inbred Strains Mitogens/isolation & purification Muramidase Nocardia/immunology Polysaccharides, Bacterial/analysis Spleen/cytology Thymus Gland/abnormalities,cytology Tissue Extracts
Chemicals
Adjuvants, Immunologic Amino Acids Amino Sugars Lipids Mitogens Polysaccharides, Bacterial Tissue Extracts Muramidase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ciorbaru R
Adam A
Petit J F
Lederer E
Bona C
Chedid L
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15 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.11.2.257-264.1975
Published
1975-02-00
Pages
257-64
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC415054
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.11.2.257-264.1975 PMC Full Text View journal details
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