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PMID: 1089601 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Test for enterotoxigenic Escherichia coli using Y-1 adrenal cells in miniculture.

Infection and immunity ·Vol. 11 ·No. 2 ·1975-02-00 ·Pages 334-6

Sack DA, Sack RB

Abstract

A rapid, potentially clinically useful test for detection of enterotoxigenic Escherichia coli is described. Whole bacterial cultures of enterotoxigenic E. coli, when briefly exposed to Y1 adrenal cells in tissue miniculture, effect a rounding response in the tissue culture that can be discerned at 18 to 24 h. The tissue culture technique agreed with the rabbit ileal loop in all 58 enterotoxigenic and 52 non-enterotoxigenic E. coli strains tested.

MeSH Terms
Adrenal Glands/cytology Animals Culture Techniques/methods Enterotoxins/analysis Escherichia coli/isolation & purification Ileum/immunology Rabbits
Chemicals
Enterotoxins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sack D A
Sack R B
References (12)
12 references, click to expand
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    J Infect Dis. 1974 Jan;129(1):1-9 PMID: 4148810
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    Infect Immun. 1974 Mar;9(3):500-5 PMID: 4593468
  8. Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture.
    Science. 1974 Jan 25;183(4122):334-6 PMID: 4587905
  9. Antitoxin responses to infections with enterotoxigenic Escherichia coli.
    J Infect Dis. 1974 Mar;129(3):330-5 PMID: 4593243
  10. Plasmid-associated enterotoxin production in a strain of Escherichia coli isolated from humans.
    Infect Immun. 1972 Apr;5(4):622-4 PMID: 4564682
  11. Toxigenic Escherichia coli: a cause of infantile diarrhea in Chicago.
    N Engl J Med. 1972 Oct 19;287(16):791-5 PMID: 4560518
  12. Production of vascular permeability factor by enterotoxigenic Escherichia coli isolated from man.
    Infect Immun. 1973 Nov;8(5):725-30 PMID: 4584048
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.11.2.334-336.1975
Published
1975-02-00
Pages
334-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC415066
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.11.2.334-336.1975 PMC Full Text View journal details
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