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PMID: 1089800 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Process of infection with bacteriophage phiX174. XXXVII. RNA metabolism in phiX174-infected cells.

Journal of virology ·Vol. 15 ·No. 1 ·1975-01-00 ·Pages 151-60

Clements JB, Sinsheimer RL

Abstract

The RNA produced in vivo from bacteriophage phiX174 DNA has been analyzed by polyacrylamide-agarose gel electrophoresis and sedimentation in dimethyl sulfoxide gradients, and the results of Hayashi and Hayashi (1970) have been confirmed and extended. An efficient procedure for recovery of RNA from gels, followed by a hybridization assay, has indicated the presence in infected cells of 18 distinct RNA species with sizes up to and greater than the unit (viral) length. The sizes of phiX mRNA's were similar irrespective of whether material was analyzed on gels or in dimethyl sulfoxide gradients. When virus-induced RNA was detected by a double-label method, seven additional low-molecular weight species were observed on gels and the resolution of dimethyl sulfoxide gradients was enhanced. The present results lend support to aspects of the model of Hayashi and Hayashi (1970) for the generation of these discrete mRNA species; an alternative model is also discussed.

MeSH Terms
Coliphages/growth & development,metabolism DNA Viruses Escherichia coli/metabolism Molecular Weight RNA, Bacterial/biosynthesis RNA, Messenger/biosynthesis RNA, Viral/biosynthesis Virus Replication
Chemicals
RNA, Bacterial RNA, Messenger RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Clements J B
Sinsheimer R L
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38 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.15.1.151-160.1975
Published
1975-01-00
Pages
151-60
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354429
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.15.1.151-160.1975 PMC Full Text View journal details
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