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PMID: 10899118 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Coupling ion specificity of chimeras between H(+)- and Na(+)-driven motor proteins, MotB and PomB, in Vibrio polar flagella.

The EMBO journal ·Vol. 19 ·No. 14 ·2000-07-17 ·Pages 3639-48

Asai Y, Kawagishi I, Sockett RE, Homma M

Abstract

We have shown that a hybrid motor consisting of proton-type Rhodobacter sphaeroides MotA and sodium-type VIBRIO: alginolyticus PomB, MotX and MotY, can work as a sodium-driven motor in VIBRIO: cells. In this study, we tried to substitute the B subunits, which contain a putative ion-binding site in the transmembrane region. Rhodobacter sphaeroides MotB did not work with either MotA or PomA in Vibrio cells. Therefore, we constructed chimeric proteins (MomB), which had N-terminal MotB and C-terminal PomB. MomB proteins, with the entire transmembrane region derived from the H(+)-type MotB, gave rise to an Na(+) motor with MotA. The other two MomB proteins, in which the junction sites were within the transmembrane region, also formed Na(+) motors with PomA, but were changed for Na(+) or Li(+) specificity. These results show that the channel part consisting of the transmembrane regions from the A and B subunits can interchange Na(+)- and H(+)-type subunits and this can affect the ion specificity. This is the first report to have changed the specificity of the coupling ions in a bacterial flagellar motor.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/chemistry,genetics,metabolism Cations, Monovalent/metabolism,pharmacology Flagella/drug effects,metabolism Genotype Hydrogen/metabolism,pharmacology Lithium/metabolism,pharmacology Locomotion/drug effects Membrane Proteins/genetics,metabolism Molecular Motor Proteins/metabolism Molecular Sequence Data Mutation Protons Recombinant Fusion Proteins/chemistry,genetics,metabolism Rhodobacter sphaeroides/genetics Sequence Alignment Sodium/metabolism,pharmacology Sodium Channels/chemistry,genetics,metabolism Substrate Specificity Vibrio/drug effects,genetics,metabolism
Chemicals
Bacterial Proteins Cations, Monovalent Membrane Proteins Molecular Motor Proteins MotA protein, Bacteria MotB protein, Bacteria PomA protein, Vibrio PomB protein, Vibrio Protons Recombinant Fusion Proteins Sodium Channels Hydrogen Lithium Sodium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Asai Y
Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-Ku, Nagoya 464-8602, Japan.
Kawagishi I
Sockett R E
Homma M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.14.3639
Published
2000-07-17
Pages
3639-48
Language
English
Region
England
NLM ID
8208664
PMCID
PMC313984
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/19.14.3639 PMC Full Text View journal details
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