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PMID: 10899130 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcriptional termination and coupled polyadenylation in vitro.

The EMBO journal ·Vol. 19 ·No. 14 ·2000-07-17 ·Pages 3770-7

Yonaha M, Proudfoot NJ

Abstract

Using a coupled, in vitro transcription and polyadenylation system we have investigated the molecular mechanism of transcriptional termination by RNA polymerase II (PolII). We showed previously that specific G-rich sequences pause transcription and then activate polyadenylation. We show that physiological pause sites activate polyadenylation in our in vitro system. We also investigate the mechanism of PolII transcriptional termination, and show that these transcripts are either directly released from the transcription complex or are 3' end processed while still attached to the complex. We also show that 3' product (generated by cleavage/polyadenylation) remains associated with the transcription complex, but is rapidly degraded on it.

MeSH Terms
Complement C2/genetics DNA/genetics,metabolism DNA-Binding Proteins Humans Oligodeoxyribonucleotides/genetics,metabolism Poly A/genetics,metabolism RNA Polymerase II/metabolism RNA Processing, Post-Transcriptional/genetics RNA, Messenger/genetics,metabolism Response Elements/genetics Sarcosine/analogs & derivatives,pharmacology Templates, Genetic Terminator Regions, Genetic/genetics Transcription Factors/genetics,metabolism Transcription, Genetic/drug effects,genetics
Chemicals
Complement C2 DNA-Binding Proteins Oligodeoxyribonucleotides RNA, Messenger Transcription Factors c-MYC-associated zinc finger protein Poly A sarkosyl DNA RNA Polymerase II Sarcosine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yonaha M
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
Proudfoot N J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.14.3770
Published
2000-07-17
Pages
3770-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC313970
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/19.14.3770 PMC Full Text View journal details
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