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PMID: 10906143 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Synaptotagmin regulation of coated pit assembly.

The Journal of biological chemistry ·Vol. 275 ·No. 40 ·2000-10-06 ·Pages 30916-24

von Poser C, Zhang JZ, Mineo C, Ding W, Ying Y, Sudhof TC, Anderson RG

Abstract

Synaptotagmins bind clathrin AP-2 with high affinity via their second C(2) domain, which indicates they are involved in coated pit function. We now report that expression of synaptotagmins lacking either the second C(2) domain or the entire cytoplasmic region potently inhibit endocytosis. Inhibition was dependent on two intramembrane cysteine residues that were found to be essential for synaptotagmin oligomerization. Cells expressing the wild-type, but not the mutant, truncated synaptotagmin fragment had a reduced number of clathrin-coated pits. These results suggest that the formation of synaptotagmin multimers is an important step in the regulation of coated pit assembly.

MeSH Terms

Adaptor Protein Complex alpha Subunits Adaptor Proteins, Vesicular Transport Calcium-Binding Proteins Cell Line Coated Pits, Cell-Membrane/metabolism,physiology,ultrastructure Cysteine/chemistry,genetics Electrophoresis, Polyacrylamide Gel Endocytosis Fluorescent Antibody Technique Genes, Dominant Genetic Vectors HeLa Cells Humans Immunohistochemistry Membrane Glycoproteins/genetics,metabolism,physiology Membrane Proteins/chemistry,genetics,metabolism Models, Genetic Mutagenesis, Site-Directed Nerve Tissue Proteins/genetics,metabolism,physiology Plasmids/metabolism Protein Structure, Tertiary Proto-Oncogene Proteins c-myc/metabolism Recombinant Proteins/metabolism Synaptotagmins Transfection

Chemicals

Adaptor Protein Complex alpha Subunits Adaptor Proteins, Vesicular Transport Calcium-Binding Proteins Membrane Glycoproteins Membrane Proteins Nerve Tissue Proteins Proto-Oncogene Proteins c-myc Recombinant Proteins Synaptotagmins Cysteine

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

von Poser C

Departments of Cell Biology, Biochemistry, and Molecular Genetics and The Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75235, USA.

Zhang J Z

Mineo C

Ding W

Ying Y

Sudhof T C

Anderson R G

Article Info

Journal

The Journal of biological chemistry

Abbr.

J Biol Chem

ISSN

0021-9258

DOI

10.1074/jbc.M005559200

Published

2000-10-06

Pages

30916-24

Language

English

Region

United States

NLM ID

2985121R

Subset

Grants

NHLBI NIH HHS · HL 20948 · United States

External Links

PubMed source DOI: 10.1074/jbc.M005559200 View journal details

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