Complementation in cytoplasmic petite mutants of yeast to form respiratory competent cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 1 ·1975-01-00 ·Pages 372-5

Clark-Walker GD, Miklos GL

Abstract

Complementation has been observed in cytoplasmic respiratory deficient yeast cells (petites) to yield respiratory competent diploids. This successful demonstration depended on the use of spontaneous petites of recent origin and on crosses involving all possible apirwise combinations between the many different petite isolates of opposite mating type. The possibility of deletion of a single unique region of yeast mitochondrial DNA as the initial lesion in petite formation has been eliminated by using strains isogenic for their mitochondrial DNA.

MeSH Terms

Crosses, Genetic Cytoplasm/metabolism DNA, Mitochondrial/metabolism Genetic Complementation Test Genotype Mutation Recombination, Genetic Saccharomyces cerevisiae/metabolism Transcription, Genetic

Chemicals

DNA, Mitochondrial

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Clark-Walker G D

Miklos G L

References (15)

15 references, click to expand

EXTRANUCLEAR TRANSMISSION IN YEAST HETEROKARYONS.
Proc Natl Acad Sci U S A. 1957 Oct 15;43(10):919-23 PMID: 16590109
[Complementation between respiratory deficient mutants of Saccharomyces cerevisiae: establishment and regulation of respiration in the zygotes and their near descendants].
Genetics. 1965 Jul;52(1):75-98 PMID: 5857600
Use of snail digestive juice in isolation of yeast spore tetrads.
J Bacteriol. 1959 Aug;78:292 PMID: 14407563
[Genetic nature of mutants lacking respiratory enzymes in the B-11 strain of baker's yeast].
Heredity (Edinb). 1950 Dec;4(3):337-51 PMID: 14802990
Mitochondrial genetics, circular DNA and the mechanism of the petite mutation in yeast.
Genet Res. 1974 Aug;24(1):43-57 PMID: 4611825
Nature of the base sequence conserved in the mitochondrial DNA of a low-density petite.
Biochim Biophys Acta. 1973 Jul 13;312(3):441-57 PMID: 4579629
Mitochondrial genetics. 3. Recombined molecules of mitochondrial DNA obtained from crosses between cytoplasmic petite mutants of Saccharomyces cerevisiae: physical and genetic characterization.
Mol Gen Genet. 1973;123(1):51-65 PMID: 4580084
Recombination in yeast mitochondrial DNA.
J Supramol Struct. 1972;1(2):145-52 PMID: 4569478
Size distribution of circular DNA from petite-mutant yeast lacking rho DNA.
Eur J Biochem. 1973 Jan 15;32(2):263-7 PMID: 4569073
Isolation of circular DNA from a mitochondrial fraction from yeast.
Proc Natl Acad Sci U S A. 1972 Feb;69(2):388-92 PMID: 4551142
Expression of the mitochondrial genome in HeLa cells. II. Evidence for complete transcription of mitochondrial DNA.
J Mol Biol. 1971 Jan 28;55(2):251-67 PMID: 5548607
Cytoplasmic DNA from petite colonies of Saccharomyces cerevisiae: a hypothesis on the nature of the mutation.
Science. 1969 Mar 21;163(3873):1331-3 PMID: 5765112
Ethidium bromide induced mutation of yeast mitochondria: complete transformation of cells into respiratory deficient non-chromosomal "petites".
Biochem Biophys Res Commun. 1968 Feb 15;30(3):232-9 PMID: 5647224
Mitochondrial DNA from yeast "petite" mutants: specific changes in buoyant density corresponding to different cytoplasmic mutations.
Biochem Biophys Res Commun. 1966 Jul 20;24(2):218-24 PMID: 5965229
Respiration-deficient mutants of yeast. I. Genetics.
Genetics. 1963 Mar;48:375-85 PMID: 13977171

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.72.1.372

Published

1975-01-00

Pages

372-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC432307

Subset

External Links

PubMed source DOI: 10.1073/pnas.72.1.372 PMC Full Text View journal details

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Complementation in cytoplasmic petite mutants of yeast to form respiratory competent cells.

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