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PMID: 10913099 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Demonstration of a novel glycolytic pathway in the hyperthermophilic archaeon Thermococcus zilligii by (13)C-labeling experiments and nuclear magnetic resonance analysis.

Journal of bacteriology ·Vol. 182 ·No. 16 ·2000-08-00 ·Pages 4632-6

Xavier KB, da Costa MS, Santos H

Abstract

The operation of a novel glycolytic pathway was demonstrated in nongrowing cells of Thermococcus zilligii by analysis of the isotopic enrichment in the end products derived from fermentation of (13)C-labeled glucose. The new pathway involved the formation of formate, derived from C-1 in glucose, via cleavage of a six-carbon carboxylic acid.

MeSH Terms

Carbon Isotopes Fermentation Glucose/metabolism Glycolysis Hexokinase/metabolism Isotope Labeling Magnetic Resonance Spectroscopy Phosphofructokinase-1/metabolism Thermococcus/growth & development,metabolism

Chemicals

Carbon Isotopes Hexokinase Phosphofructokinase-1 Glucose

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Xavier K B

Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, 2780-156 Oeiras, Portugal.

da Costa M S

Santos H

References (13)

13 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/JB.182.16.4632-4636.2000

Published

2000-08-00

Pages

4632-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC94637

Subset

External Links

PubMed source DOI: 10.1128/JB.182.16.4632-4636.2000 PMC Full Text View journal details

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Demonstration of a novel glycolytic pathway in the hyperthermophilic archaeon Thermococcus zilligii by (13)C-labeling experiments and nuclear magnetic resonance analysis.

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