Representational difference analysis of Neisseria meningitidis identifies sequences that are specific for the hyper-virulent lineage III clone.

FEMS microbiology letters ·Vol. 188 ·No. 2 ·2000-07-15 ·Pages 111-4

Bart A, Dankert J, van der Ende A

Abstract

Neisseria meningitidis may cause meningitis and septicemia. Since the early 1980s, an increased incidence of meningococcal disease has been caused by the lineage III clone in many countries in Europe and in New Zealand. We hypothesized that lineage III meningococci have specific DNA sequences, providing an opportunity to facilitate epidemiological studies by detecting lineage III isolates rapidly. Applying representational difference analysis on one lineage III tester strain and two non-lineage III driver strains, we identified three lineage III-specific sequences, probably part of a single locus encoding a restriction modification system. A PCR based on one of these sequences identified lineage III meningococcal isolates with a sensitivity of 100% and a specificity of 93%, which is superior to the serological identification of lineage III isolates.

MeSH Terms

Blotting, Southern DNA, Bacterial/analysis,genetics False Positive Reactions Humans Meningococcal Infections/epidemiology,microbiology Molecular Epidemiology Molecular Sequence Data Neisseria meningitidis/genetics,pathogenicity Netherlands/epidemiology Polymerase Chain Reaction/methods Sensitivity and Specificity Virulence/genetics

Chemicals

DNA, Bacterial

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Bart A

Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands. [email protected]

Dankert J

van der Ende A

Article Info

Journal

FEMS microbiology letters

Abbr.

FEMS Microbiol Lett

ISSN

0378-1097

DOI

10.1111/j.1574-6968.2000.tb09179.x

[email protected]

Published

2000-07-15

Pages

111-4

Language

English

Region

England

NLM ID

7705721

Subset

Databases

GENBANK

AF119281, AF119282, AF119283, AF119284, AF119285, AF119286, AF119287, AF119288, AF119289, AF119290, AF119291, AF119292, AF119293, AF119294, AF119295, AF119296

External Links

PubMed source DOI: 10.1111/j.1574-6968.2000.tb09179.x View journal details

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Representational difference analysis of Neisseria meningitidis identifies sequences that are specific for the hyper-virulent lineage III clone.

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