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PMID: 10915624 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dependence of stem cell fate in Arabidopsis on a feedback loop regulated by CLV3 activity.

Science (New York, N.Y.) ·Vol. 289 ·No. 5479 ·2000-07-28 ·Pages 617-9

Brand U, Fletcher JC, Hobe M, Meyerowitz EM, Simon R

Abstract

The fate of stem cells in plant meristems is governed by directional signaling systems that are regulated by negative feedback. In Arabidopsis thaliana, the CLAVATA (CLV) genes encode the essential components of a negative, stem cell-restricting pathway. We used transgenic plants overexpressing CLV3 to show that meristem cell accumulation and fate depends directly on the level of CLV3 activity and that CLV3 signaling occurs exclusively through a CLV1/CLV2 receptor kinase complex. We also demonstrate that the CLV pathway acts by repressing the activity of the transcription factor WUSCHEL, an element of the positive, stem cell-promoting pathway.

MeSH Terms

Arabidopsis/cytology,genetics,growth & development,metabolism Arabidopsis Proteins Cell Differentiation Cell Division Down-Regulation Feedback Gene Expression Regulation, Plant Genes, Plant Homeodomain Proteins/genetics,metabolism In Situ Hybridization Membrane Proteins/metabolism Meristem/cytology,metabolism Mutation Phenotype Plant Proteins/genetics,metabolism Plants, Genetically Modified Protein Serine-Threonine Kinases RNA, Messenger/genetics,metabolism RNA, Plant/genetics,metabolism Receptor Protein-Tyrosine Kinases/metabolism Signal Transduction Stem Cells/cytology,metabolism Transcription Factors Transgenes

Chemicals

AT2G27250 protein, Arabidopsis Arabidopsis Proteins CLV2 protein, Arabidopsis Homeodomain Proteins Membrane Proteins Plant Proteins RNA, Messenger RNA, Plant Transcription Factors UFO protein, Arabidopsis WUSCHEL protein, Arabidopsis Receptor Protein-Tyrosine Kinases CLV1 protein, Arabidopsis Protein Serine-Threonine Kinases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Brand U

Institut für Entwicklungsbiologie, Universität zu Köln, Gyrhofstrabetae 17, D-50923 Köln, Germany.

Fletcher J C

Hobe M

Meyerowitz E M

Simon R

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.289.5479.617

Published

2000-07-28

Pages

617-9

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.289.5479.617 View journal details

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Dependence of stem cell fate in Arabidopsis on a feedback loop regulated by CLV3 activity.

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