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PMID: 1091626 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Isolation and partial characterization of three Escherichia coli mutants with altered transfer ribonucleic acid methylases.

Journal of bacteriology ·Vol. 122 ·No. 1 ·1975-04-00 ·Pages 257-65

Marinus MG, Morris NR, Söll D, Kwong TC

Abstract

Seven transfer ribonucleic acid (tRNA) methylase mutants were isolated from Escherichia coli K-12 by examining the ability of RNA prepared from clones of unselected mutagenized cells to accept methyl groups from S-adenosylmethionine catalyzed by crude enzymes from wild-type cells. Five of the mutants had an altered uracil-tRNA methylase; consequently their tRNA's lacked ribothymidine. One mutant had tRNA deficient in 7-methylguanosine, and one mutant contained tRNA lacking 2-thio-5-methylaminomethyluridine. The genetic loci of the three tRNA methylase mutants were distributed over the E. coli genome. The mutant strain deficient in 7-methylguanosine biosynthesis showed a reduced efficiency in the suppression of amber mutations carried by T4 or lambda phages.

MeSH Terms
Chromatography, Affinity Chromatography, Thin Layer Chromosome Mapping Electrophoresis Escherichia coli/enzymology,isolation & purification Genes Guanosine/analogs & derivatives,biosynthesis Methyltransferases/metabolism Mutagens Mutation Nitrosoguanidines RNA, Bacterial/metabolism RNA, Ribosomal/metabolism RNA, Transfer/metabolism Recombination, Genetic S-Adenosylmethionine/metabolism Thymidine/analogs & derivatives,biosynthesis Uridine/analogs & derivatives,biosynthesis
Chemicals
Mutagens Nitrosoguanidines RNA, Bacterial RNA, Ribosomal Guanosine S-Adenosylmethionine RNA, Transfer Methyltransferases Thymidine Uridine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Marinus M G
Morris N R
Söll D
Kwong T C
References (28)
28 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.122.1.257-265.1975
Published
1975-04-00
Pages
257-65
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC235665
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.122.1.257-265.1975 PMC Full Text View journal details
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