Abstract
Rapid analysis of microbial communities has proven to be a difficult task. This is due, in part, to both the tremendous diversity of the microbial world and the high complexity of many microbial communities. Several techniques for community analysis have emerged over the past decade, and most take advantage of the molecular phylogeny derived from 16S rRNA comparative sequence analysis. We describe a web-based research tool located at the Ribosomal Database Project web site (http://www.cme.msu.edu/RDP/html/analyses. html) that facilitates microbial community analysis using terminal restriction fragment length polymorphism of 16S ribosomal DNA. The analysis function (designated TAP T-RFLP) permits the user to perform in silico restriction digestions of the entire 16S sequence database and derive terminal restriction fragment sizes, measured in base pairs, from the 5' terminus of the user-specified primer to the 3' terminus of the restriction endonuclease target site. The output can be sorted and viewed either phylogenetically or by size. It is anticipated that the site will guide experimental design as well as provide insight into interpreting results of community analysis with terminal restriction fragment length polymorphisms.
MeSH Terms
Bacteria/classification,genetics,isolation & purification
DNA, Bacterial/analysis,genetics
DNA, Ribosomal/analysis,genetics
Databases, Factual
Ecosystem
Internet
Phylogeny
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 16S/genetics
Sewage/microbiology
Software
Soil Microbiology
Chemicals
DNA, Bacterial
DNA, Ribosomal
RNA, Ribosomal, 16S
Sewage
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Marsh T L
Center for Microbial Ecology, Michigan State University, East Lansing, Michigan 48824, USA.
[email protected]
Saxman P
Cole J
Tiedje J
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