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PMID: 1093003 Published · ppublish English Journal Article

The selective iodination of yeast phenylalanine transfer RNA with 125-I.

Molecular biology reports ·Vol. 2 ·No. 1 ·1975-03-00 ·Pages 65-72

Batey IL, Brown DM

Abstract

Yeast tRNA-Phe has been labelled with 125-I under conditions which conserve the tertiary structure. Significant labelling was only found to occur on specific cytidines in single stranded regions, while other cytidines in single stranded regions and all those in the double stranded region underwent iodination to a very small extent. The pattern obtained from iodine labelling satisfies the conformation of a model recently proposed for this tRNA.

MeSH Terms
Binding Sites Chromatography, Gel Cytidine/analysis Iodine Radioisotopes Isotope Labeling/methods Nucleic Acid Conformation Oligonucleotides/analysis Pancreas/enzymology Phenylalanine RNA, Transfer/isolation & purification Ribonucleases Saccharomyces cerevisiae/analysis
Chemicals
Iodine Radioisotopes Oligonucleotides Phenylalanine Cytidine RNA, Transfer Ribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Batey I L
Brown D M
References (17)
17 references, click to expand
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Article Info
Journal
Molecular biology reports
Abbr.
Mol Biol Rep
ISSN
0301-4851
DOI
10.1007/BF00357299
Published
1975-03-00
Pages
65-72
Language
English
Region
Netherlands
NLM ID
0403234
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00357299 View journal details
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