The secondary structure of E. coli ribosomes and ribosomal RNA's: a spectrophotometric approach.

Nucleic acids research ·Vol. 2 ·No. 3 ·1975-03-00 ·Pages 373-81

Araco A, Belli M, Giorgi C, Onori G

Abstract

The thermal denaturation spectra of E. coli 50s and 30s ribosomal sub units and of their isolated RNA's were studied over the wavelength range of 2300-3000 Angstrom. It was possible to fit the experimental denaturation spectra with super positions of the reference spectra for denaturation of A - U and G - C base pairs derived from model polyribonucleotides. The coefficients of these linear combinations were used to calculate the fractions of A - U and G - C base pairs in the samples. It was found that the helical content of the RNA's inside the subunits is smaller than that of the isolated RNA's, thus suggesting that proteins may affect the secondary structure of RNA in the ribosome.

MeSH Terms

Escherichia/analysis Escherichia coli/ultrastructure Magnesium Nucleic Acid Conformation Nucleic Acid Denaturation RNA, Bacterial/analysis RNA, Ribosomal/analysis Ribosomes/analysis,ultrastructure Spectrophotometry, Ultraviolet Temperature

Chemicals

RNA, Bacterial RNA, Ribosomal Magnesium

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Araco A

Belli M

Giorgi C

Onori G

References (12)

12 references, click to expand

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Thermal denaturation of ribosomes.
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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/2.3.373

Published

1975-03-00

Pages

373-81

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342843

Subset

External Links

PubMed source DOI: 10.1093/nar/2.3.373 PMC Full Text View journal details

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The secondary structure of E. coli ribosomes and ribosomal RNA's: a spectrophotometric approach.

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