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PMID: 1093173 Published · ppublish English Journal Article

Regulation of RNA polymerase synthesis in Escherichia coli: a mutant unable to synthesize the enzyme at 43 degrees.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 3 ·1975-03-00 ·Pages 911-5

Oeschger MP, Berlyn MK

Abstract

We report the isolation of a mutant of E. coli in which the capacity to synthesize RNA polymerase (EC 2.7.7.6) (the beta and beta' subunits) is rapidly lost at 43 degrees. The mutation has no effect on the stability or activity of the polymerase itself. The mutation is recessive and is closely linked to the rif locus (the structural gene for the beta subunit). Using strains carrying the mutation, we have shown that polymerase is present in excess in rapidly growing E. coli cells.

MeSH Terms
Coliphages DNA-Directed RNA Polymerases/biosynthesis Drug Resistance, Microbial Escherichia coli/drug effects,enzymology Genes Genes, Recessive Genetic Linkage Kinetics Mutation Rifamycins/pharmacology Temperature Transduction, Genetic
Chemicals
Rifamycins DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Oeschger M P
Berlyn M K
References (27)
27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.3.911
Published
1975-03-00
Pages
911-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432431
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.3.911 PMC Full Text View journal details
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