Home LiteratureArticle Details
PMID: 1093180 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Site specific recA--independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 4 ·1975-04-00 ·Pages 1373-7

Kopecko DJ, Cohen SN

Abstract

A recA-independent recombinational event is described which results in insertion of an entire plasmid genome at a unique site of another plasmid, and coincident excision of a precisely defined DNA segment originally present at the point of the insertion. The resulting recombinant molecules subsequently can undergo site-specific translocation of their component segments or inversion of their original DNA sequence orientation. The events observed entail nonreciprocal exchange of genetic material, and involve a discrete nucleotide sequences that is duplicated in rotationally symmetrical reverse orientation on plasmid DNA (i.e., inverted repeat; palindrome).

MeSH Terms
Base Sequence Centrifugation, Density Gradient DNA, Bacterial/metabolism Electrophoresis Escherichia coli/metabolism,ultrastructure Extrachromosomal Inheritance Genes Microscopy, Electron Molecular Weight Mutation Plasmids Recombination, Genetic Transformation, Genetic Translocation, Genetic
Chemicals
DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kopecko D J
Cohen S N
References (24)
24 references, click to expand
  1. Episome-mediated transfer of drug resistance in Enterobacteriaceae. V. Spontaneous segregation and recombination of resistance factors in Salmonella typhimurium.
    J Bacteriol. 1962 Sep;84:422-30 PMID: 13999114
  2. Replication and transcription of eukaryotic DNA in Escherichia coli.
    Proc Natl Acad Sci U S A. 1974 May;71(5):1743-7 PMID: 4600264
  3. Non-chromosomal antibiotic resistance in bacteria. II. Molecular nature of R-factors isolated from Proteus mirabilis and Escherichia coli.
    J Mol Biol. 1970 Jun 28;50(3):671-87 PMID: 4920496
  4. Sequence of a repressor-binding site in the DNA of bacteriophage lamda.
    Nature. 1974 Aug 2;250(465):394-7 PMID: 4854243
  5. Molecular structure of bacterial plasmids.
    Bacteriol Rev. 1972 Sep;36(3):361-405 PMID: 4345849
  6. Abnormal metabolic response to ultraviolet light of a recombination deficient mutant of Escherichia coli K12.
    J Mol Biol. 1966 Aug;19(2):442-54 PMID: 4291028
  7. Palindromes in chromosomes.
    J Mol Biol. 1974 Mar 25;84(1):115-38 PMID: 4208606
  8. IS2, a genetic element for turn-off and turn-on of gene activity in E. coli.
    Mol Gen Genet. 1974;132(4):265-89 PMID: 4610339
  9. A method for selective cloning of eukaryotic DNA fragments in Escherichia coli by repeated transformation.
    Mol Gen Genet. 1974;134(2):133-41 PMID: 4617159
  10. Recircularization and autonomous replication of a sheared R-factor DNA segment in Escherichia coli transformants.
    Proc Natl Acad Sci U S A. 1973 May;70(5):1293-7 PMID: 4576014
  11. Molecular nature of R-factor deoxyribonucleic acid isolated from Salmonella typhimurium minicells.
    J Bacteriol. 1973 Jun;114(3):1328-35 PMID: 4576405
  12. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors.
    J Mol Biol. 1973 Apr 5;75(2):235-55 PMID: 4580676
  13. Molecular and genetic studies of an R factor system consisting of independent transfer and drug resistance plasmids.
    J Bacteriol. 1973 Nov;116(2):699-709 PMID: 4583247
  14. The nucleotide sequence of the lac operator.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3581-4 PMID: 4587255
  15. Construction of biologically functional bacterial plasmids in vitro.
    Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4 PMID: 4594039
  16. Restriction and modification of DNA: enzymes and substrates. Introductory remarks.
    Fed Proc. 1974 May;33(5):1125-7 PMID: 4599005
  17. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  18. Nonchromosomal antibiotic resistance in bacteria. V. Isolation and characterization of R factor mutants exhibiting temperature-sensitive repression of fertility.
    J Bacteriol. 1972 Jun;110(3):1082-8 PMID: 4555403
  19. The 3'-terminal nucleotide sequences of bacteriophage lambda DNA.
    Proc Natl Acad Sci U S A. 1973 Apr;70(4):1151-5 PMID: 4515613
  20. Recombination and complementation between R factors in Escheichia coli K 12.
    Genet Res. 1971 Dec;18(3):287-97 PMID: 4945740
  21. Non-chromosomal antibiotic resistance in bacteria. 3. Isolation of the discrete transfer unit of the R-factor R1.
    Proc Natl Acad Sci U S A. 1970 Oct;67(2):510-6 PMID: 4943172
  22. Studies on resistance transfer factor deoxyribonucleic acid in Escherichia coli.
    J Bacteriol. 1970 Oct;104(1):340-4 PMID: 4919749
  23. A restriction enzyme from Hemophilus influenzae. II.
    J Mol Biol. 1970 Jul 28;51(2):393-409 PMID: 5312501
  24. Characterization of a transfer factor associated with drug resistance in Salmonella typhimurium.
    Nature. 1965 Nov 27;208(5013):843-9 PMID: 5331112
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.4.1373
Published
1975-04-00
Pages
1373-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432536
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.4.1373 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2