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PMID: 10934258 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Antisense suppression of potassium channel expression demonstrates its role in maturation of the action potential.

Vincent A, Lautermilch NJ, Spitzer NC

Abstract

A developmental increase in delayed rectifier potassium current (I(Kv)) in embryonic Xenopus spinal neurons is critical for the maturation of excitability and action potential waveform. Identifying potassium channel genes that generate I(Kv) is essential to understanding the mechanisms by which they are controlled. Several Kv genes are upregulated during embryogenesis in parallel with increases in I(Kv) and produce delayed rectifier current when heterologously expressed, indicating that they could encode channels underlying this current. We used antisense (AS) cRNA to test the contribution of xKv3.1 to the maturation of I(Kv), because xKv3.1 AS appears to suppress specifically heterologous expression of potassium current by xKv3.1 mRNA. The injection of xKv3.1 AS into embryos reduces endogenous levels of xKv3.1 mRNA in the developing spinal cord and reduces the amplitude and rate of activation of I(Kv) in 40% of cultured neurons, similar to the percentage of neurons in which endogenous xKv3.1 transcripts are detected. The current in these mature neurons resembles that at an earlier stage of differentiation before the appearance of xKv3.1 mRNA. Furthermore, AS expression increases the duration of the action potential in 40% of the neurons. No change in voltage-dependent calcium current is observed, suggesting that the decrease in I(Kv) is sufficient to account for lengthening of the action potential. Computer-simulated action potentials incorporating observed reductions in amplitude and rate of activation of I(Kv) exhibit an increase in duration similar to that observed experimentally. Thus xKv3.1 contributes to the maturation of I(Kv) in a substantial percentage of these developing spinal neurons.

MeSH Terms
Action Potentials/drug effects,physiology Animals Calcium Channels/drug effects,metabolism Cells, Cultured Embryo, Nonmammalian Female Kinetics Models, Neurological Neurons/cytology,drug effects,metabolism Oocytes Potassium Channels/drug effects,genetics,metabolism RNA, Antisense/pharmacology RNA, Messenger/analysis,metabolism Spinal Cord/cytology,embryology,metabolism Xenopus laevis
Chemicals
Calcium Channels Potassium Channels RNA, Antisense RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vincent A
Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093-0357, USA.
Lautermilch N J
Spitzer N C
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
0270-6474
Published
2000-08-15
Pages
6087-94
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6772606
Subset
IM
Grants
NINDS NIH HHS · NS07220 · United States
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