The nuclear import function of Smad2 is masked by SARA and unmasked by TGFbeta-dependent phosphorylation.

Amino Acid Motifs Amino Acid Sequence Biological Transport/drug effects Carrier Proteins/metabolism Cell Membrane Permeability/drug effects Cell Nucleus/drug effects,metabolism DNA-Binding Proteins/chemistry,genetics,metabolism Digitonin/pharmacology HeLa Cells Humans Immunohistochemistry Intracellular Signaling Peptides and Proteins Phosphorylation/drug effects Protein Binding/drug effects Protein Structure, Tertiary Recombinant Fusion Proteins/chemistry,metabolism Sequence Deletion Serine Endopeptidases Smad2 Protein Trans-Activators/chemistry,genetics,metabolism Transfection Transforming Growth Factor beta/pharmacology

Carrier Proteins DNA-Binding Proteins Intracellular Signaling Peptides and Proteins Recombinant Fusion Proteins SMAD2 protein, human Smad2 Protein Trans-Activators Transforming Growth Factor beta ZFYVE16 protein, human Serine Endopeptidases Digitonin